���ɷַ��������첻ͬ�����ڲ�ɺ����7����ѧ�ɷֵĺ���

Principal Components Analysis in Determination of Seven Chemical Constituents in Different Parts and Collecting Time of Sarcandra glabra by HPLC

??OBJECTIVE To develop an HPLC method for simultaneous determination of seven active constituents including neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin-3-O-??-D-glucuronide, isofraxidin, kaempferol-3-O-??-D-glucuronide and rosmarinic acid in different parts and various collecting time of Sarcandra glabra.METHODS The DIKMA Diamonsil C₁₈(4.6 mm??250 mm,5 ??m) column was used. acetonitrile(A)-0.2%phosphoric acid(B) was used as the mobile phase with gradient elution(0-10 min,5% A??15% A;11-18 min,15% A??25% A;18-25 min,25% A;25-40 min,25% A??40% A),at a flow rate of 1.0 mL??min^-1. The detection wavelength was set at 344 nm and the column temperature was maintained at 35 ??.RESULTS The calibration curves of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, quercetin-3-O-??-D-glucuronide, isofraxidin, kaempferol-3-O-??-D-glucuronide and rosmarinic acid were in good linearity over 9.32-466.00, 11.25-562.50, 10.94-547.00, 8.68-434.00, 10.48-524.00, 9.66-483.00 and 10.86-543.00 ??g??mL^-1 (r=0.999 6-0.999 9), respectively. The average recoveries (n=6)were in the range of 99.0%-101.5% and RSD were in the range of 0.85%-1.8%. The optimal parts and collecting time of Sarcandra glabra are aerial parts and from September to November according to the comprehensive evaluation.CONCLUSION The method is precise and reliable, can be used to determin the content of seven chemical constituents in Sarcandra glabra and provide a reference for collecting in cultivate area.