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Discrimination of Rhodiola crenulata by HPLC Fingerprinting and Similarity Analysis

??OBJECTIVE To establish the HPLC fingerprint of Rhodiola crenulata herbs from Sichuan plateau, and compare them with commercially available samples. METHODS RP-HPLC analysis was applied using Agilent Zorbax C₁₈ chromatographic column (4.6 mm??250 mm,5 ??m). The mobile phase A was 0.1% formic acid aqueous solution and mobile phase B was 0.1% formic acid in acetonitrile. The flow rate was 1 mL??min^-1 and the column temperature was maintained at 35 ??. The detection wavelength was set at 245 nm and injection of sample was 20 ??L. The traditional Chinese medicine fingerprint chromatogram similarity evaluation system (Version 2004A), principal component analysis, and cluster analysis were used to compare 30 Rhodiola crenulata samples from various locations based on their HPLC chromatograms. RESULTS The established HPLC fingerprint of Rhodiola crenulata was able to analyze Rhodiola crenulata from different sources. CONCLUSION The method has good repeatability and stability, and can be used for the quality management standard of Rhodiola crenulata.