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Reduction of Iron Deposition by Verbascoside in HO-1 Overexpression Rats Astrocytes

??OBJECTIVE To investigate the effect of verbascoside on iron deposition of astrocyte cell overexpressing heme oxygenase-1 gene, and to seek new drug in treating Alzheimer??s disease. METHODS Primary cultured astrocyte cells of rats were cultured and purified in vitro. With gene recombination and transfer techniques, transfect HO-1 gene into primary cultured astrocyte cells.HO-1 transfected astrocyte were then treated by different concentrations of verbascoside.Proliferation of cell was measured by CCK-8 assay and expression of HO-1 protein was analyzed by Western blotting. Iron deposition was also visualized by Perl??s Prussian blue stain. RESULTS Immunofluorescence staining showed that most of the cells were GFAP-postitive and the purity was over 95%. The expression of HO-1 protein was increased in HO-1 injury model of astrocyte. Treatment of verbascoside with variable concentration caused the decrease of HO-1 protein expression more or less and reduction of iron deposition in transfected cells(20 ??mol??L^-1reducing 47.69%,40 ??mol??L^-1 reducing 51.63%). CONCLUSION Verbascoside could enhance astrocyte cell resistance against injury induced by overexpression of heme oxygenase-1 gene.