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Simultaneous Determination of Five Components in Ermiao Pills by UHPLC with Dual-Wavelength

??OBJECTIVE To develop an ultra-high performance liquid chromatography(UHPLC) method for simultaneous determination of berberine hydrochloride, phellodendrine hydrochloride, palmatine hydrochloride, magnoflorine, and rhizoma atractylodis in Ermiao pills. METHODS The UHPLC analyses were performed on a Waters BEH C₁₈ column (2.1 mm??100 mm,1.7 ??m)eluted with acetonitrile and 0.1% phosphoric acid(each 100 mL containing 0.1 g sodium dodecyl sulfate). The flow rate was 0.3 mL??min^-1, the detection wavelength was set at 280 and 340 nm, and the column temperature was set at 30 ??. RESULTS The calibration curves of the five compositions had good linear relationship in the ranges of the tested concentrations. The precision, stability and repeatability complied with the requirements of methodology. The recoveries were between 96.8%-99.3% respectively. The RSDs were below 2.8%. CONCLUSION The developed method is simple, sensitive, rapid and accurate. This work provides helpful information for the comprehensive quality evaluation of Ermiao pills.