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Spectrum-Effect Relation of Antioxidant Activity of Flos Carthami Based on DPPH,ABTS and FRAP Assay

??OBJECTIVE To study the active ingredients of Flos Carthami by investigating the relationship between HPLC spectrum and antioxidant activity. METHODS The specific chromatogram of Flos Carthami processed by means of classical homothermal acceleration was assayed by HPLC. DPPH, ABTS and FRAP assays were established to determine the antioxidant activity of Flos Carthami. The spectrum-effect correlation was studied by partial least squares (PLS) method. RESULTS S₄, S₈, S₁₁, S₁₂, S₁₃, S₁₄, S₁₆ and S₁₇ were characteristic compounds in 27 matching characteristic chromatograms which had positive relationship with the ability of scavenging DPPH free radical. S₄, S₈, S₁₁, S₁₂, S₁₄, S₁₆, S₁₇ and S₁₈ were significantly positively related to the ability of scavenging ABTS free radical. S₄, S₈, S₁₁, S₁₂, S₁₃, S₁₄, S₁₆, S₁₇, S₁₈ and S₂₆ were significantly positively related to the ability of restoring Fe³⁺. Among the chromatographic peaks, S₄, S₈, S₁₁, S₁₂, S₁₄, S₁₆ and S₁₇ were positively related to the abilities of scavenging DPPH free radical and ABTS free radical and restoring Fe³⁺. S₁₄ was determined as hydroxysafflor yellow A. CONCLUSION The antioxidant material of Flos Carthami is based on the synergy of a variety of compounds. An as comprehensive as possible analysis of the compounds related to the pharmacological effect can reflect the quality of Flos Carthami. The study of spectrum-efficacy relationship is an effective way to determine the compounds related with pharmacological effect and control the quality of traditional Chinese medicinal materials.