˿�ʵ��׾��������ٰ��Զ��������ҩ�����е���Ҫ���о�

Investigation the Importance of Serglycinin the Drug-Resistance to the Doxorubicin in Breast Cancer

??OBJECTIVE To explore whether knockdown serglycin's expression level in MDA-MB-231 cells by transient transfection can improve the sensitivity of breast cancer cells to the doxorubicin.METHODS At first, qRT-PCR,Western-Blot and immunofluorescence were used to examine the expression level in two different cell lines, MDA-MB-231 and MCF-7. Then serglycin's expression level in MDA-MB-231 was knocked down by using transient transfection, qRT-PCR and immunofluorescence were used to test the efficiency of this approach; and then, between the NC group and the Si-SG group, MTS was used to measure the IC₅₀ of doxorubicin and the proliferation curve under the treatment of the doxorubicin. Also the numbers of colony formation in this two groups was observed when treating with different concentration of doxorubicin. RESULTS It was found that in these two cells, the expression of serglycin in MDA-MB-231 is significantly higher than MCF-7. The efficiency of knockdown in MDA-MB-231 is above 70%. In Si-SG group, the IC₅₀ and the growth curve under treatment with doxorubicin is significantly lower than the NC group. Same RESULTS can be found in the colony formation assay, when treating with the doxorubicin, the decreasing rate of colony numbers is significantly quicker in the Si-SG group than NC group. CONCLUSION Knockdown serglycin's expression level in MDA-MB-231 cells by transient transfection can improve the sensitivity to the doxorubicin.