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Effects of Elicitors on Fucoxanthin Production and ZDS Gene Expression in Phaeodactylum tricornutum

??OBJECTIVE To explore the effects of methyl jasmonic acid(MeJA), arachidonic acid(AA) and acetylsalicylic acid(ASA) on fucoxanthin production and PtZDS gene expression in Phaeodactylum tricornutum.METHODS The full length cDNA of PtZDS gene in P. tricornutum was obtained by HiSeq^TM Illumina 2000 and bioinformatics analysis. HPLC was used to determine the production of fucoxanthin in Phaeodactylum tricornutum, and RT-PCR was used to determine the expression level of PtZDS.RESULTS The total length of PtZDS gene was 1 905 bp, containing an open reading frame of 1 776 bp encoding 591 amino acids.The deduced amino acid sequence analysis showed that PtZDS protein contained a typical amino oxidase domain, NAD(P)-binding Rossmann-like domain and a chloroplastic transit peptide sequence.The phylogenetic analysis demonstrated that PtZDS was homologous with Thalassiosira pseudonana CCMP1335(76%). Under the treatment of 0.1 mg·L^-1 AA, 25 mg·L^-1 ASA and 100 μmol·L^-1 MeJA, the highest yield of unit cell fucoxanthin content was achieved.Upon the observation of PtZDS regulation expression, the expression level of PtZDS reached a peak value under the treatment of 0.1 mg·L^-1 AA, 50 μmol·L^-1 MeJA and 10 mg·L^-1 ASA.CONCLUSION The expression of PtZDS gene has certain relationship with the fucoxanthin synthesis of P. tricornutum. Under the treatment of 100 μmol·L^-1 MeJA, the ability of synthetic fucoxanthinis the strongest in P. tricornutum.