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| 碱性pH对浮游状态和生物膜状态粪肠球菌活性的影响 | |
| 引用本文： | Yan PF,Liang JP,Jiang YT. 碱性pH对浮游状态和生物膜状态粪肠球菌活性的影响[J]. 上海口腔医学, 2012, 21(1): 6-8 |
| 作者姓名： | Yan PF Liang JP Jiang YT |
| 作者单位： | 上海交通大学医学院附属第九人民医院·口腔医学院牙体牙髓科上海市口腔医学重点实验室 |
| 基金项目： | 国家自然科学基金(30801291)~~ |
| 摘 要： | 目的:比较生物膜状态与浮游状态下粪肠球菌对碱的耐受性。方法:制备浮游状态和生物膜状态的粪肠球菌细胞,用pH值分别为7、8、9、10、11和12的培养液作用2h,利用MTT比色法比较2种状态下细菌细胞活性变化。采用SAS6.12软件包对数据进行统计学分析。结果:低碱性环境(pH值7～9)对粪肠球菌的生长无明显影响,高碱性环境(pH值>10)下存活细菌的比例明显减少;高pH环境下,生物膜状态的粪肠球菌存活细菌比例显著高于浮游状态下细菌的存活比例。结论:粪肠球菌对碱性环境具有强耐受性,形成生物膜是粪肠球菌抵抗高碱性环境的一个重要原因。 |
| 关 键 词： | 粪肠球菌 生物膜 pH值 |
The influence of different alkaline pH conditions on Enterococcus faecalis in planktonic and biofilm mode |
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| Yan Pei-fang,Liang Jing-ping,Jiang Yun-tao. The influence of different alkaline pH conditions on Enterococcus faecalis in planktonic and biofilm mode[J]. Shanghai journal of stomatology, 2012, 21(1): 6-8 | |
| Authors: | Yan Pei-fang Liang Jing-ping Jiang Yun-tao |
| Affiliation: | Department of Endodontics, Shanghai Jiao Tong University School of Medicine, Shanghai, China. yanpeifang@126.com |
| Abstract: | PURPOSE: To study the influence of different pH conditions on Enterococcus faecalis(E.faecalis) in planktonic and biofilm mode.METHODS: E.faecalis were prepared in planktonic and biofilm mode and cultured in TSB mediums 2 hours at pH 7,8,9,10,11 and 12.MTT assays were applied to evaluate the survival rate of bacterial cells in different pH value.SAS 6.12 software package was used for statistical analysis.RESULTS: Mild alkaline mediums(pH7-9) had no effect on cell vitality of E.faecalis and high alkaline condition(pH>10) led to significant declines of survival rate of cells.The biofilm cells of E.faecalis were more alkaline tolerant than corresponding planktonic cells.CONCLUSION: Biofilm formation is an important step in the development of alkaline tolerance of E.faecalis. |
| Keywords: | Enterococcus faecalis Biofilm pH value |
| 本文献已被 CNKI PubMed 等数据库收录！ | |