Evaluation of photodynamic therapy on fibroblast viability and cytokine production |
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| Institution: | 1. Department of Restorative Dentistry, Discipline of Endodontics, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil;2. Department of Restorative Dentistry, Discipline of Endodontics, Dental School, University of São Paulo, São Paulo, SP, Brazil;3. Optics Group, Physics Institute of São Carlos, University of São Paulo, São Carlos, SP, Brazil;4. Department of Basic Science, Discipline of Biochemistry, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil;5. Department of Basic Science, Discipline of Pharmacology, Araçatuba School of Dentistry, São Paulo State University, Araçatuba, SP, Brazil;1. Department of Endodontics, School of Dentistry, São Paulo State University (Unesp), Universidade Estadual Paulista, Araçatuba, São Paulo, Brazil;2. Department of Clinic and Surgery and Animal Reproduction, Veterinary Medicine, São Paulo State University (Unesp), Araçatuba, São Paulo, Brazil;1. Department of Dentistry, Federal University of Vales do Jequitinhonha e Mucuri, Diamantina, Minas Gerais, Brazil;2. Postgraduate program in Dentistry, University CEUMA, Rua Josué Montelo, No 1, Renascença II, São Luís, Maranhão CEP: 65075-120, Brazil;3. Departament of dentistry, State University of Montes Claros, Montes Claros, Minas Gerais, Brazil;4. Department of Microbiology, Institute of Biological Sciences, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil;1. Department of Oral Diagnosis and Laser in Dentistry Research Group, Faculty of Dentistry, Khon Kaen University, Khon Kaen, 40002, Thailand;2. Department of Clinical Chemistry, Faculty of Associated Medical Science, Khon Kaen University, Khon Kaen, 40002, Thailand;3. Department of Physics, Faculty of Sciences, Khon Kaen University, Khon Kaen, 40002, Thailand;1. Department of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia;2. Department of Restorative Dental Sciences, College of Dentistry, King Saud University, Riyadh 11545, Saudi Arabia;3. Department of Prosthetic Dental Sciences, College Of Dentistry, King Saud University, Riyadh, Saudi Arabia;4. Department Of Clinical Dental Sciences, College Of Dentistry, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia;5. Department of Restorative Dental Sciences, College of Dentistry, King Saud University, And Engineer Abdullah Bugshan Research Chair For Dental And Oral Rehabilitation, Riyadh, Saudi Arabia;6. Department of Prosthetic Dental Sciences, College of Dentistry, King Saud University; Engineer Abdullah Bugshan Research Chair For Dental and Oral Rehabilitation, College of Dentistry, King Saud University, Riyadh, Saudi Arabia;1. Department of Prosthetic Dental Sciences, College of Dentistry, King Saud University, Riyadh, Saudi Arabia;2. Faculty of Dentistry, Department of Restorative Dentistry, University of Malaya, Kuala Lumpur, Malaysia;3. Division of General Dentistry, Eastman Institute for Oral Health, University of Rochester, NY 14620, USA;4. Dental Practice, 13 Xenofontos Strt., Athens, Greece |
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| Abstract: | BackgroundThe aim of this study was to evaluate the effects of photodynamic therapy with curcumin (PDT) comparatively to 5% sodium hypochlorite (NaOCl) and saline solution on cell viability and cytokine (IL-1β and IL-6) production by mouse fibroblasts.MethodsSixty seconds of pre-irradiation time with curcumin 500 mg/L and Led wavelength (λ) 480 nm, 72 J cm2, for 300 s was used for PDT. Solutions were diluted in culture medium DMEM (1 × 104 cells) and placed into 24-well cell culture plates with mouse fibroblasts L-929. Culture medium was used as control. After 6, 24 and 48 h, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay (MTT) was used to evaluate the cell viability and the supernatant was collected for cytokine evaluation using enzyme-linked immunosorbent assay (ELISA). The results were statistically analyzed by ANOVA and BonFerroni correction (p < 0.05) for MTT and Kruskal–Wallis test and Dunn (p < 0.05) for ELISA.ResultsPDT and saline solution presented low cytotoxic effect similar to the control group (p > 0.05) while 5% NaOCl was more cytotoxic than PDT (p < 0.05) in all periods of time. All materials similarly expressed IL-1β and IL-6 regardless to the experimental period (p < 0.05).ConclusionsPDT with curcumin was not cytotoxic to L929 fibroblasts differently from 5% NaOCl. In all groups occurred similar expression of IL-1β and IL-6. |
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| Keywords: | Endodontic treatment Root canal irrigants Photodynamic therapy Cytotoxicity Cytokine |
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