腹腔感染大鼠肠屏障功能损伤研究

目的:观察大鼠腹腔感染后肠屏障功能损伤的相关性改变。方法:将健康成年大鼠40只随机分为对照组(仅行单纯剖腹手术)和腹腔感染组(采用盲肠结扎穿孔法制作腹腔感染模型),于术后12、24、36和48 h取材,每个时间点取8只大鼠。处死后,距回盲部10 cm处取回肠行常规病理检查,同时测定肠黏膜绒毛高度、肠上皮损伤指数和各相应时间点血浆D-乳酸含量。结果:腹腔感染后12 h,大鼠肠绒毛高度明显降低(P<0.01),肠黏膜损伤评分增加,血浆D-乳酸含量明显升高(P<0.01);24 h肠损伤达到高峰,肠黏膜损伤评分最大,血浆D-乳酸含量明显增高(P<0.05)。随后,D-乳酸水平逐渐下降,但48 h组损伤程度仍显著高于对照组(P<0.01)。大鼠血浆D-乳酸水平与小肠绒毛高度呈负相关(r=-0.696,P<0.01),与损伤指数呈显著正相关(r=0.489,P<0.01)。结论:在腹腔感染状态下,肠屏障功能严重受损,感染后24 h可达高峰。

Study on injury of intestinal barrier function in rats with intraperitoneal infection

Objective: To observe the correlated changes of the gut barrier when damaged by intraperitoneal infection in rats.Methods: Forty healthy adult Wistar rats were randomly divided into control group(celiotomy ones only) and groups of 12,24,36,48 hours post-operation of abdominal infection respectively(by cecal ligation and puncture).Each group contained 8 rats.After the rats were anaesthetized,2 cm of the ileum tissue was sampled for optical observations,including histological examination,determination of villous thickness and evaluation of mucosal damage index.D-lactate in plasma was measured at 4 consecutive time points.Results: Intestinal barrier began to change significantly 12 h after the infection,including lower villous thickness(P<0.01),higher mucosal damage index and higher lever of D-lactate in plasma(P<0.01).The damage reached the peak at 24 h: the lowest villus height,the maximal mucosal injury score and the highest lever of D-lactate.Then the level of D-lactate began to fall but remained still serious(P<0.01).There was direct relationship between the D-lactate level and villous thickness(r=-0.696,P<0.01).And D-lactate level also had a significant correlation with mucosal damage index(r=0.489,P<0.01).Conclusion: Intestinal mucosal barrier is subjected serious damage and the damage reaches the peak at 24 h post-operation in rats with intraperitoneal infection.