酪氨酸激酶抑制剂抑制骨髓瘤细胞的增殖及黏附

目的 观察酪氨酸激酶抑制剂ST1571对骨髓瘤细胞系RPM18226细胞增殖、黏附以及黏附诱导耐药的影响。方法该试验采用MTT法检测ST1571对骨髓瘤细胞增殖的影响；流式细胞仪检测对细胞周期的影响；结晶紫染色法分析RPM18226细胞与纤连蛋白（fibronectin，FN）的黏附率；MTT法检测ST1571对瘤细胞耐药的影响；结果ST1571明显抑制KPM18226细胞的增殖，24、48和72hIC50值分别为（34．52±2．31）、（28．46±1．58）和（25．74±2．44）μmol／L。25μmol／L ST1571处理24h，G1期细胞由55．8％增加至72．4‰S期细胞由34．8％减至15．6％。KPM18226细胞与FN黏附1、6和12h，其黏附率分别为（43．71％±2．18％）、（55．63％±1．56％）和（63．42％±2．46％）；非抑制浓度ST1571（20μmol／L）处理1、6和12h后黏附率分别降为（15．12±1．04）％、（17．58±1．32）％和（17．24±1．59）％；组间差异有显著性（P〈0．05）；阿霉毒作用后，FN黏附组细胞IC50值[（1．46±O．04）μmol／L]显著高于BSA组[（0．78±0．03）μmol／L]（P〈0．05）；FN联合ST1571组IC50值[（0．81±0．05）μmol／L]与BSA联合ST1571组[（0．74±0．02）μmol／L]相比差异无显著性（P〉0．05），但却低于FN组（P〈0．05）。结论ST1571能抑制KPM18226细胞的增殖、并可降低KPM18226细胞与FN的黏附率及逆转黏附介导的阿霉素耐药。

Tyrosine-kinase inhibitor inhibits proliferation and adhesion of multiple myeloma cell line RPMI8226 cells

[Objective] To investigate the effects of tyrosine-kinase inhibitor STI571 on RPMI8226 cell proliferation, and adhesion of RPMI8226 cells to fibronectin, cell adhesion mediated adriamycin resistance. [Methods] By means of MTT assays, the proliferation of RPMI8226 cells was determined. Flow cytometry was used to observe cell cycle. By means of crystal violet staining and MTT assays, the adhesion of RPMI8226 cells to fibronectin and drug resistance mediated by cell adhesion were determined. [Results] The prol...