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| Effect of Certain Toxicants on Gonadotropin—Induced Ovarian Non—Esterified Cholesterol Depletion and Steroidogenic Enzyme Stimulaiton of the Common Carp Cyprinus carpio in vitro | |
| 引用本文： | DILIPMUKHERJEE DHRITIGUHA 等. Effect of Certain Toxicants on Gonadotropin—Induced Ovarian Non—Esterified Cholesterol Depletion and Steroidogenic Enzyme Stimulaiton of the Common Carp Cyprinus carpio in vitro[J]. Biomedical and environmental sciences : BES, 1992, 5(2): 92-98 |
| 作者姓名： | DILIPMUKHERJEE DHRITIGUHA 等 |
| 作者单位： | [1]EndorinologyLaboratory,DepartmentofZoology,UniversityofKalyani,Kalyani741235,India [2]EndorinologyLabora,DepartmentofZoology,UniversityofKalyani,Kalyani741235,India |
| 摘 要： | Isolated ovarian tissues from the common carp,Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin,namely phenol,sulfide, mercuric chloride and cadmium chloride,on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes,△^5-3β-HSD and 17β-HSD activity.Stage Ⅱ ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both.Safe doses of above mentioned toxicants when added separaetely to stage Ⅱ ovarian tissue with oLH(1μg/incubation)gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited.Esterified cholesterol remained almost unaltered,Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately. |
| 关 键 词： | 鲤鱼 毒物 促性腺素 卵巢 非酯化胆固醇耗尽 产固醇酶刺激 环境污染物 繁殖 |
Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro. |
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| D Mukherjee,D Guha,V Kumar. Effect of certain toxicants on gonadotropin-induced ovarian non-esterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro.[J]. Biomedical and environmental sciences : BES, 1992, 5(2): 92-98 | |
| Authors: | D Mukherjee D Guha V Kumar |
| Affiliation: | Department of Zoology, University of Kalyani, India. |
| Abstract: | Isolated ovarian tissues from the common carp, Cyprinus carpio were incubated in vitro to obtain a discrete effect of four common toxicants of industrial origin, namely phenol, sulfide, mercuric chloride and cadmium chloride, on gonadotropin-induced alteration of nonesterified and esterified cholesterol and steroidogenic enzymes, delta 5-3 beta-HSD and 17 beta-HSD activity. Stage II ovarian tissue containing 30-40% mature oocytes were shown to be most responsive to gonadotropins in depleting only nonesterified cholesterol moiety and stimulating the activity of both. Safe doses of above mentioned toxicants when added separately to stage II ovarian tissue with oLH (1 microgram/incubation) gonadotropin-induced depletion of nonesterified cholesterol and gonadotropin-induced stimulation of the activity of both enzymes was significantly inhibited. Esterified cholesterol remained almost unaltered. Findings clearly indicate the impairment of gonadotropin induced fish ovarian steroidogenesis by the four toxicants separately. |
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