实验性糖尿病鼠脑微血管病变与氧化应激反应的研究

目的建立实验性2型糖尿病（T2DM）鼠模型并探讨脑组织和血管病变与脑组织及血液中醛糖还原酶（AR）活性、超氧阴离子（O2^-·）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-PX）活性改变及其在T2DM发生发展中的相互关系。方法组织切片观察脑组织和微血管病理改变；紫外分光光度法及化学比色法检测血清O2^-·、GSH—PX、SOD、CAT活性；NADPH减少法测定脑组织和血清AR活性。结果实验性T2DM鼠脑微血管和脑组织病理形态学改变明显；脑组织和血清AR活性显著高于正常对照组（P〈0．05）；O2^-·活力显著高于正常对照组（P〈0．05）、SOD、GSH．Px活性显著低于正常对照组（P〈0．05）；T2DM鼠H组SOD活性显著低于L组（P〈0．05）。结论实验性T2DM鼠脑组织出现病理变化与血液和脑组织中氧化与抗氧化物质异常改变密切相关，在诸多因素中，SOD活性降低可能是起主导作用的因素。

Study on Relation Between Microangiopathy and Oxidative Stress in Diabetic Rats Brain

Objective To establish an animal model similar to human type 2 diabetis and evaluate 2^-· , SOD, CAT, AR activity in microangiopathy of diabetic rat brain. Methods Observe micrangium pathological change in diabetic rats brain ; The activity of AR in the blood and brain tissue was measured by NADPH decreasing test method. The activity of 2^-· , GSH-Px, SOD and CAT was measured by biochemistry test method. Results There are micrangium and nerve tissue pathological changes in brain of diabetic rats. The activity of AR in the blood and brain tissue of diabetic group is higher than that in control group significantly. The activity of 2^-· in diabetis group was higher compared with that of control group（ P 〈0.05）. The activity of SOD and GSH-Px was lower compared with that of control group（ P 〈0.05）. The activity of SOD in H group is lower compared with L group（ P 〈 0.05）. Conclusion The pathological change in brain tissue of diabetic rat is related to oxidation and anti-oxidation change in blood and brain tissue and SOD may be a more important factor than others.