γ-干扰素释放分析T-SPOT.TB在结核性疾病中的诊断价值

目的评价γ-干扰素释放分析T-SPOT.TB检测在结核性疾病中的诊断价值。方法采用T-SPOT.TB试剂盒对疑诊或待排结核患者外周血中释放γ-干扰素的结核分枝杆菌特异性T淋巴细胞进行检测。结果γ-干扰素释放分析T-SPOT.TB检测在结核性疾病阳性检出率为83.3%(20/24),明显高于结核菌素试验(tuberculin skin test,TST)的41.7%(10/24)、涂片找抗酸杆菌的26.7%(4/15)、分枝杆菌分离培养的22.2%(2/9),差异有统计学意义(P<0.05)。γ-干扰素释放分析T-SPOT.TB检测诊断结核性疾病敏感性、特异性分别为83.3%、96.4%,显著优于结核菌素试验。结论γ-干扰素释放分析T-SPOT.TB检测是诊断结核的快速敏感方法,在结核性疾病诊断中有重要价值。

Diagnostic value of interferon-gamma release assay T-SPOT.TB for tuberculosis

Objective To evaluate the diagnostic value of an interferon-gamma release assay T-SPOT.TB in tuberculosis.Methods T-SPOT.TB assay reagent was employed to detect Mycobacterium tuberculosis antigen specific T cells in the peripheral blood samples from the patients with suspected tuberculosis.Results The positive detection rate was 83.3%(20/24) by interferon-gamma release assay T-SPOT.TB was significantly higher than 41.7%(10/24) by tuberculin test,26.7%(4/15) in smear for acid-fast bacillus and 22.2%(2/9) in mycobacteria separation culture with statistical differences(P<0.05).The sensitivity and specificity of interferon-gamma release assay T-SPOT.TB were 83.3% and 96.4%,which were significantly higher than those of tuberculin test with statistical differences(P<0.05).Conclusions Interferon-gamma release assay T-SPOT.TB is rapid and high-sensitive test for diagnosing tuberculosis.