|
|||||
|
|
| Effects of anti-CXCR4 monoclonal antibody 12G5 on proliferation and apoptosis of human acute myelocytic leukemia cell line HL-60 | |
| 引用本文： | 魏立,孔佩艳,史占忠,曾东风,陈幸华,常城,彭贤贵,张怡,刘红. Effects of anti-CXCR4 monoclonal antibody 12G5 on proliferation and apoptosis of human acute myelocytic leukemia cell line HL-60[J]. 中国人民解放军军医大学学报, 2007, 22(1): 17-22. DOI: 10.1016/S1000-1948(07)60004-5 |
| 作者姓名： | 魏立 孔佩艳 史占忠 曾东风 陈幸华 常城 彭贤贵 张怡 刘红 |
| 作者单位： | Department of Hematology Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China，Department of Hematology，Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China，Department of Hematology，Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China，Department of Hematology，Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China，Department of Hematology，Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China，Department of Hematology，Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China，Department of Hematology，Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China，Department of Hematology，Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China，Department of Hematology，Xinqiao Hospital，Third Military Medical University，Chongqing 400037，China |
| 摘 要： | Objective:To investigate the expression of CXCR, on HL-60 cell line and the proliferation, apoptosis of HL-60 cell line cocultured with bone marrow stromal cells, so as to assess the possibility of 12G5. an anti-CXCR4 monoclonal antibody, in eradicating the minimal residual disease. Methods:The activity of SDF-1 was inhibited by 10μg/ml 12G5. After treatment with 12G5. the status of adhesion was observed, and the adhesion rates, apoptosis and cell cycles were detected after 24 h of treatment. Cell growth rates were measured by trypan blue exclusion. Cell growth curve was plotted, and the expression of PCNA and apoptosis related protein including PCNA, Bcl-2 and Fas were detected with immunohis-tochemical technique. Results :(1) There was middling degree expression of CXCR4 on HL-60 membrane. From 0 h to 6 h, as the time of 12G5 incubation along, the expression of CXCR4 decreased gradually. (2) After treatment for 24 h, the adhesion rates in the experiment group and the control were (39. 4±7. 9)% and (51. 4±5. 9)%, respectively. (3)After treatment for 24 h, the percentage of HL-60 cells in G0/G1 phase were (55. 21±4. 9)%, and that in S phase and G2/M phase were (30. 40±4. 1)% and (14. 39±5.2)%, respectively, with the corresponding proportions being (44. 67±2. 2) % , (45. 30±3. 7)% . and (10. 03±2. 6)% in the control. (4) The percentage of apoptotic HL-60 cells was (8. 95±1. 7)% in the experiment group, compared to (3. 97±2. 4)% in the control. (5)The survival rates of HL-60 cells decreased markedly at 48 h to 96 h, and the proliferation slowed down at this time duration. (6)The expression of PCNA and Bcl-2 down-regulated significantly, but the Fas protein expression was up-regulated. Conclusion :12G5 could inhibit the capability of adhesion and proliferation of HL-60 cells and it can induce more cells to enter G0/G1 phase and promote apoptosis. It may be helpful by inhibiting the bioactivity of SDF-1 with 12G5 in the therapy of marrow residual disease. |
| 关 键 词： | 急性粒细胞性白血病 肿瘤细胞系 SDF-1/CXCR4 单克隆抗体 细胞增殖 细胞凋亡 |
| 收稿时间： | 2006-07-20 |
| 修稿时间： | 2006-11-10 |
Effects of anti-CXCR4 monoclonal antibody 12G5 on proliferation and apoptosis of human acute myelocytic leukemia cell line HL-60 |
|
| WEI Li,KONG Pei-yan,SHI Zhan-zhong,ZENG Dong-feng,CHEN Xing-hua,CHANG Cheng,PENG Xian-gui,ZHANG Yi,LIU Hong. Effects of anti-CXCR4 monoclonal antibody 12G5 on proliferation and apoptosis of human acute myelocytic leukemia cell line HL-60[J]. Journal of Medical Colleges of PLA(China), 2007, 22(1): 17-22. DOI: 10.1016/S1000-1948(07)60004-5 | |
| Authors: | WEI Li KONG Pei-yan SHI Zhan-zhong ZENG Dong-feng CHEN Xing-hua CHANG Cheng PENG Xian-gui ZHANG Yi LIU Hong |
| Affiliation: | 1. College of Food Science and Technology, Laboratory of Quality & Safety Risk Assessment for Aquatic Products on Storage and Preservation, Ministry of Agriculture, Shanghai Engineering Research Center of Aquatic-Product Processing & Preservation, Shanghai Ocean University, No. 999, Huchenghuan Road, Shanghai 201306, PR China;2. The Institute for Biomedical Engineering and Nano Science, Tongji University School of Medicine, Shanghai 200092, PR China;1. Institute for Neuroscience, The University of Texas at Austin, Austin, TX, USA;2. Department of Psychology, The University of Texas at Austin, Austin, TX, USA;3. Division of Pharmacology and Toxicology, The University of Texas at Austin, Austin, TX, USA;1. University of Strathclyde Business School, Department of Management Science, 199 Cathedral Street, Glasgow G4 0QU, Scotland, UK;2. Worcester Polytechnic Institute, Department of Social Science and Policy Studies, 100 Institute Road, Worcester, MA 01609, USA |
| Abstract: | ObjectiveTo investigate the expression of CXCR4 on HL-60 cell line and the proliferation. apoptosis of HL-60 cell line cocultured with bone marrow stromal cells, so as to assess the possibility of 12G5, an anti-CXCR4 monoclonal antibody, in eradicating the minimal residual disease.MethodsThe activity of SDF-1 was inhibited by 10 μg/ml 12G5. After treatment with 12G5, the status of adhesion was observed, and the adhesion rates, apoptosis and cell cycles were detected after 24 h of treatment. Cell growth rates were measured by trypan blue exclusion. Cell growth curve was plotted, and the expression of PCNA and apoptosis related protein including PCNA, Bcl-2 and Fas were detected with immunohis-tochemical technique.Results(1) There was middling degree expression of CXCR4 on HL-60 membrane. From 0 h to 6 h, as the time of 12G5 incubation along, the expression of CXCR4 decreased gradually. (2) After treatment for 24 h, the adhesion rates in the experiment group and the control were (39.4 ± 7.9) and (51.4±5.9)%, respectively. (3) After treatment for 24 h, the percentage of HL-60 cells in G. G. phase were (55.21±4.9)%, and that in S phase and G2/M phase were (30.40±4.1)%, and (14.39 = 5.2)%, respectively, with the corresponding proportions being (44.67±2.2)%, (45.30±3.7)%, and (10.03±2.6)% in the control. (4) The percentage of apoptotic HL-60 cells was (8.95±1.7)% in the experiment group, compared to (3.97±2.4)% in the control. (5) The survival rates of HL-60 cells decreased markedly at 48 h to 96 h, and the proliferation slowed down at this time duration. (6) The expression of PCNA and Bcl-2 down-regulated significantly, but the Fas protein expression was up-regulated.Conclusion12G5 could inhibit the capability of adhesion and proliferation of HL-60 cells and it can induce more cells to enter G0/G1 phase and promote apoptosis. It may be helpful by inhibiting the bloactivity of SDF-1 with 12G5 in the therapy of marrow residual disease. |
| Keywords: | SDF-1/CXCR4 monoclonal antibody acute leukemia proliferation apoptosis drug resistance marrow residual disease |
| 本文献已被 CNKI 万方数据 ScienceDirect 等数据库收录！ | |