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Ki-ras-2 in acute lymphoblastic leukemia cells with chromosome change at 12p12 |
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| Authors: | Kazuma Ohyashiki Junko H. Ohyashiki Alan J. Kinniburgh Keisuke Toyama Misao Ito Jun Minowada Avery A. Sandberg |
| Affiliation: | a Department of Genetics and Endocrinology, Roswell Park Memorial Institute, Buffalo, NY, USA b Department of Human Genetics, Roswell Park Memorial Institute, Buffalo, NY, USA c Department of Internal Medicine, Tokyo Medical College, Tokyo, Japan d Fujisaki Cell Center, Hayahibara Biochemical Laboratories Inc., Okayama, Japan. |
| Abstract: | Cytogenetic and molecular investigations were performed on a pre-B-lymphoblastic acute leukemic cell line (NALM-6). The NALM-6 cells contained a del(5)(q32) and an ins(12)(p12;?), chromosomal material of unknown origin being inserted between subbands 12p12.1 and 12p12.2. Chromosomal in situ hybridization using a 3.0-kb c-Ki-ras-2 probe showed a significant accumulation of grains on the proximal portion of the inserted chromosomal material (12p12.1), as well as on the normal chromosome #12 with a peak at 12p11-p12. The signal intensity obtained after hybridization of the c-Ki-ras-2 specific probe to the NALM-6 cells DNA is comparable with the intensity of the signal after hybridization of the same probe with the control cell line (MC/B) DNA. The findings indicate that the c-Ki-ras-2 gene is neither amplified nor transposed in the NALM-6 cells. |
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