基于药效筛选和正交试验优化温肺降浊方的提取工艺

目的通过药效、急毒实验筛选温肺降浊方的提取工艺,并以正交试验进行优化,确定温肺降浊方的最佳提取工艺。方法对不同工艺样品进行急性毒性实验,并以血管性痴呆(VD)大鼠的学习和记忆能力的改善程度为药效指标,综合急毒与药效实验的结果,筛选出最佳工艺方案;以多个成分含量和出膏率为指标,采用正交试验筛选溶剂倍量、提取时间和提取次数的最优水平,并进行验证。结果急毒实验结果显示所有小鼠均健康存活,未见明显毒性反应。药效实验结果表明白附片、党参、炙甘草混合水提,三七、干姜、酒大黄混合醇提为温肺降浊方的最佳提取方案;最优提取工艺为白附片先煎2h,再入党参、炙甘草加10倍水加热回流提取3次,每次1 h;三七、干姜、酒大黄加6倍70%乙醇加热回流提取3次,每次1.5 h。结论温肺降浊方能明显改善VD模型大鼠的学习和记忆能力,优选的工艺条件合理可行,为温肺降浊方的后续开发提供依据。

Optimization of extraction process of Wenfei Jiangzhuo Recipe based on pharmacodynamic screening and orthogonal experiment

Objective To screen the extraction process of Wenfei Jiangzhuo Recipe (WJR) by pharmacodynamics and acute toxicity test, and to optimize it by orthogonal experiment to determine the best extraction process of WJR.Methods Acute toxicity test was carried out on different WJR samples. The improvement of learning and memory of vascular dementia (VD) rats was used as the efficacy index, and the results of the acute toxicity and efficacy experiments determined the best process plan. Using contents and yield rate as index, orthogonal test was used to screen the amount of solvent, extraction time and extraction times.Results The results of acute toxicity test showed that all mice survived without obvious toxicity. The results of pharmacodynamic test showed that the best extraction plan of WJR was as follows:Extracting white prepared lateral root of Aconite, Codonopsis Radix, and Glycyrrhizae Radix et Rhizoma Praeparata cum Melle with water, and Notoginseng Radix et Rhizoma, Zingiberis Rhizoma, and wine processing Rhei Radix et Rhizoma with ethanol. The optimized extraction technology were as follows:The former were 10-fold water reflux extraction for three times and 1 h each time, with white prepared lateral root of Aconite first reflux 2 h; The latter were 6-fold 70% ethanol reflux extraction three times and each time 1.5 h.Conclusion WJR can significantly improve the learning and memory ability of VD model rats. The optimized extraction conditions are reasonable and feasible, and can provide the basis for the subsequent development of WJR.