| 贵州不同产地粗毛淫羊藿药用植物遗传多样性及亲缘关系的ISSR分析 |
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| 引用本文： | 石建明,李牡丹,杨鹏,陈兴银,彭昌琴,刘鑫,关萍.贵州不同产地粗毛淫羊藿药用植物遗传多样性及亲缘关系的ISSR分析[J].中草药,2018,49(13):3122-3126. |
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| 作者姓名： | 石建明 李牡丹 杨鹏 陈兴银 彭昌琴 刘鑫 关萍 |
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| 作者单位： | 贵州大学生命科学学院;广东省国基教育发展研究院 |
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| 基金项目： | 贵州省社会发展攻关课题（201303137）；中央高校基本业务费一般项目（XDJK2016C006） |
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| 摘 要： | 目的对贵州不同产地粗毛淫羊藿样品的遗传多样性和亲缘关系进行分析。方法利用Pop Gene 32软件及NTsys2.10e软件分析贵州5个不同产地粗毛淫羊藿的遗传多样性及亲缘关系,并根据UPGMA法,构建亲缘关系树状图。结果从100个随机引物中筛选出13条多态性稳定、条带清晰的引物,结果表明,共扩增出211个位点,从物种水平上看,共有205个多态性位点,多态性百分率(PPB)为97.16%,Shannon’s信息指数(I)为0.455 7,Nei’s基因多样性指数(H_e)为0.297 3,有效等位基因数(N_e)为1.490 2,5个居群间总的遗传多样性(H_t)为0.297 3,而居群内的遗传多样性(H_s)为0.207 5,表明粗毛淫羊藿的遗传变异主要存在于群体内,种群内的遗传分化大于种群间。利用UPGMA法构建的居群遗传距离聚类树,表明,5个居群被分为2支,其中来自安顺、花溪、高坡的3个居群聚在一支,来自龙里和雷山的2个居群共同聚为一支。结论 ISSR分子标记技术可以用于贵州不同产地的粗毛淫羊藿植物的遗传多样性和亲缘关系分析。
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| 关 键 词： | 粗毛淫羊藿 ISSR 遗传多样性 亲缘关系 UPGMA法 |
| 收稿时间： | 2017/12/16 0:00:00 |
ISSR analysis of genetic diversity and relationship among medicinal plants of Epimedium acuminatum from different habitats in Guizhou Province |
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| SHI Jian-ming,LI Mu-dan,YANG Peng,CHEN Xing-yin,PENG Chang-qin,LIU Xin and GUAN Ping.ISSR analysis of genetic diversity and relationship among medicinal plants of Epimedium acuminatum from different habitats in Guizhou Province[J].Chinese Traditional and Herbal Drugs,2018,49(13):3122-3126. |
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| Authors: | SHI Jian-ming LI Mu-dan YANG Peng CHEN Xing-yin PENG Chang-qin LIU Xin and GUAN Ping |
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| Institution: | College of Life Sciences, Guizhou University, Guiyang 550025, China,Guangdong Institute of National Basic Education Development and Research, Guangzhou 511400, China,College of Life Sciences, Guizhou University, Guiyang 550025, China,College of Life Sciences, Guizhou University, Guiyang 550025, China,College of Life Sciences, Guizhou University, Guiyang 550025, China,College of Life Sciences, Guizhou University, Guiyang 550025, China and College of Life Sciences, Guizhou University, Guiyang 550025, China |
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| Abstract: | Objective To analyze the genetic diversity and relationship of Epimedium acuminatum from different habitats in Guizhou Province. Methods PopGene 32 software and NTsys2.10e software was used to analyze the genetic diversity and phylogenetic relationship of five different habitats in Guizhou Province, and phylogenetic tree was constructed according to the UPGMA method. Results A total of 13 polymorphic and clear bands were screened from 100 random primers showed that amplified 211 bands, of which 205 were polymorphic from the level of species, the percentage of polymorphic bands (PPB) was 97.16%. The Shannon''s information index (I) was 0.455 7; Nei''s gene diversity index (He) was 0.297 3; The effective number (Ne) of alleles was 1.490 2; The total genetic diversity (Ht) of five populations was 0.297 3, while the population genetic diversity within the group (Hs) was 0.207 5, indicated that the genetic variation mainly existed within populations, genetic differentiation within population than that of population between. The population genetic distance clustering was built by UPGMA. The results showed that five populations were divided into two branches, three populations from Anshun, Huaxi, and Gaopo clustered into one branch, and the other two populations from Longli and Leishan populations clustered into one branch. Conclusion ISSR molecular marker technique can be used to analyze the genetic diversity and phylogenetic relationship of Epimedium acuminatum from different habitats in Guizhou Province. |
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| Keywords: | Epimedium acuminatum Franch ISSR genetic diversity genetic relationship UPGMA method |
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