土茯苓黄酮类成分一测多评方法的建立及含量变化规律研究

目的建立土茯苓Smilax glabra中新落新妇苷、落新妇苷、新异落新妇苷、异落新妇苷和黄杞苷的一测多评测定方法(QAMS)并研究不同生长年限土茯苓中各成分含量的动态变化规律。方法采用高效液相色谱法,以土茯苓中的落新妇苷为内参物,计算该成分与新落新妇苷、新异落新妇苷、异落新妇苷和黄杞苷的相对校正因子,用获得的相对校正因子计算各指标成分的含量并与外标法的测定结果进行对比,考察方法的准确性和可行性。采用建立的QAMS法测定不同生长年限土茯苓中各指标成分的含量,研究其动态变化规律。结果共测定不同生长年限样品5批23份,各指标成分一测多评法与外标法计算结果偏差均小于1.0%。各指标成分含量与生长年限具有一定相关性,落新妇苷的含量与生长年限呈负相关,新落新妇苷、新异落新妇苷和异落新妇苷的含量与生长年限呈正相关,两者的变化趋势相反。结论建立的QAMS法准确、可靠,可用于土茯苓中5个成分的定量分析。生长年限对土茯苓中4种落新妇苷类成分含量均具一定影响。

Content changes regularity of flavonoids in Smilax glabra by QAMS method

Objective To establish a method of quantitative analysis of multi-components by single-marker (QAMS) for simultaneously determination of neoastilbin, astilbin, neoisoastilbin, isoastilbin, and engeletin in Smilax glabra, and research the tendency of content changes in different growth years by QAMS. Methods An HPLC method was established to determine the relative correction factors of four other flavonoids by using astilbin as the internal reference standard. Then the method was used to determine the various content of five flavonoids in different growth years and validate the feasibility and accuracy by comparing the content results determined by the external standard method with QAMS. The dynamic change regularity of flavonoids in S. glabra was investigated. Results A total of 23 samples from five batches in different growth years were simultaneously determined by external standard method and QAMS, the results deviation were all less than 1.0%. The content of five flavonoids in different growth years was different, astilbin decreased with the increase of growth years, neoastilbin, neoisoastilbin and isoastilbin had the reverse trend. Conclusion The established QAMS method is feasible and accurate for the simultaneous determination of five flavonoids in S. glabra. Growth years have a certain impact on the content of each component.