运动性蛋白尿与肾脏细胞凋亡及氧自由基代谢关系的研究

目的 :观察大鼠不同强度运动后肾脏细胞凋亡及氧自由基变化与运动性蛋白尿之间的关系 ,以期进一步阐明运动性蛋白尿的发生机制。方法 :36只雄性SD大鼠随机分为安静对照组、中等强度运动组和大强度运动组。运动后 2小时取材 ,采用原位缺口末端标记 (TUNEL)和流式细胞术分别检测大鼠肾脏凋亡细胞及Bax、Bcl- 2基因的表达 ;同时检测大鼠肾脏组织超氧化物歧化酶 (SOD)、谷胱甘肽过氧化物酶 (GSH -Px)、丙二醛 (MDA)含量及运动后各实验组大鼠尿蛋白含量。结果 :运动后大强度组肾脏凋亡细胞显著增加 ,凋亡调控基因Bax/Bcl - 2比值上升 (P <0 0 1) ,尿蛋白含量显著升高 ,反映肾脏组织细胞抗氧化能力的SOD及GSH -Px活性显著下降 ,而MDA含量显著升高。结论 :大强度运动造成肾脏细胞凋亡及氧自由基增加可能参与了运动性蛋白尿的发生过程。

Studies on the Association of the Exertional Proteinuria with Apoptosis and Oxidative Free Radical Metabolism of Renal Cells

Objective To explore the mechanism of exertional proteinuria. Methods 36 male Sprague-Dawley rats were randomly divided into sedentary control group, middle-intensity and high-intensity exercise group. We sampled rats kidney tissue and urine post exercise at 120min. Apoptosis and Bax?Bcl-2 genes expression of renal cell were investigated with TUNEL and flow cytometry, respectively. The concentrations of superoxide dismutase (SOD), malondialdehyde (MDA) and Glutathione peroxidase (GSH-Px) as well as urinary protein were measured at the same time. Results Apoptosis cells and urinary protein in high-intensity training group increased significantly in concomitant with the increase of Bax/Bcl-2 ratio ( P < 0 01). It was also observed that concentration of SOD and GSH-Px in high-intensity exercise group decreased while that of MDA increased significantly. Conclusion Caused by high intensity exercise, the increase of apoptosis and accumulation of oxygen free radical in renal cells play an important role in the occurrence of exertional proteinuria.