新生大鼠脊髓神经干细胞的分离培养及鉴定

目的　从新生大鼠的脊髓中分离培养神经干细胞并观察其增殖和分化能力。方法　采用细胞培养技术结合间接免疫荧光细胞化学法。结果　分离的细胞生长旺盛 ,单克隆化生成的细胞团 ,BrdU掺入呈强阳性。分离培养获得的细胞团呈Nestin强阳性 ,至今已在体外连续传代 8个月。培养的细胞团经 1%小牛血清诱导可分化为神经元和星形胶质细胞。结论　成功分离培养了新生大鼠脊髓神经干细胞

Isolation,Culture and Characterization of Neural Stem Cells from Newborn Rat Spinal Cords

ObjectiveTo study the isolation, culture and characterization of neural stem cells from newborn rat spinal cords. Methods Cell culture technique and indirect immunoflurenscence cytochemistry were used. Results The cells were proliferated vitally. Neurospheres achieved by single cell cloning were positive with incoporation of BrdU and Nestin immunoflurescence staining. The cells had survived and grown for 8 months in vitro. The neurospheres could be induced to differentiate into neurons and astrocytes by 1% calf serum. Conclusions Neural stem cells from newborn rat spinal cords were cultured successfully.