大鼠卵黄囊的分化及其肿瘤性转化研究

目的研究12d大鼠胚胎脏层卵黄囊(VYS)向多胚层组织分化的潜能和在逆转录病毒感染下的肿瘤性转化特征。方法在不同培养条件、移植位点的条件下,观察VYS体内外分化的改变;另外利用逆转录病毒载体将荧光蛋白基因(GFP)转染12d卵黄囊细胞,对GFP标记的转化细胞进行体内外研究。结果在不同培养条件下,均对体外培养的或体内移植的大鼠卵黄囊向三个胚层分化的进程无特异的导向性。将荧光蛋白标记卵黄囊克隆细胞接种在裸鼠皮下长出了未分化的间质细胞肉瘤。结论12d大鼠胚胎脏层卵黄囊具有向三胚层分化的潜能;逆转录病毒感染导致卵黄囊间质细胞发生肿瘤性转化。

Differentiation and Neoplastic Transformation of Visceral Yolk Sac in Rats

Objective To study the differentiation and neoplastic transformation by retrovirus infection of 12 d visceral yolk sac (VYS) of rats in vitro and in vivo. Methods Under various cultrue conditions and at different transplant sites in vivo, the differentiation of rat VYS was investigated. The VYS was transfected by retroviral vector carrying green fluorescent protein (EGFP) gene. The positive clones selected by G418 in vitro were re-implanted into syngenic rats or immunodeficiency mice in vivo. Results The VYS showed totipotency to differentiate into ectoderm, mesoderm and endoderm, but did not become committed to a particular cell lineage under whatever culture conditions or the implantion sites in the present experiment. Green fluorescent protein was successfully labelled into cloned VYS cells. When a large amount of labelled cells were innoculated s.c. into nude mice, mesenchymal neoplasm developed with GFP positive indicating VYS origin.