| 人酪氨酸羟化酶基因真核表达载体的构建及在哺乳动物细胞中的表达 |
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| 引用本文： | 崔宁,应其龙. 人酪氨酸羟化酶基因真核表达载体的构建及在哺乳动物细胞中的表达[J]. 中国临床神经科学, 1995, 3(4): 191-193 |
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| 作者姓名： | 崔宁 应其龙 |
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| 作者单位： | 上海医科大学分子遗传学研究室 200032(崔宁，宋后燕)，上海医科大学神经病学研究所 200040(应其龙，江澄川)，上海医科大学神经病学研究所 200040(唐镇生) |
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| 摘 要： | 用限制性内切酶EcoRI从pKS(-)HTH_1切下全长为1.9 kb的人酪氨酸羟化酶基因,在T_4DNA连接酶的作用下连接在真核表达载体pCDNA_3的EcoR Ⅰ位点,构建成重组质粒pcD-NA_3HTH_1,该质粒转染COS-7细胞,免疫荧光组织化学染色证实酪氨酸羟化酶在其中的表达。
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| 关 键 词： | 酪氨酸羟化酶 酪氨酸羟化酶cDNA 质粒 COS-7细胞 转染 |
Construction of Eukaryotic Expression Vector Containing Human HTH1 cDNA Transcriptional Unit and Its Expressionin Mammalian Cells |
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| Cui Ning,Song Hou-Yan Molecular Genetics Laboratory. Construction of Eukaryotic Expression Vector Containing Human HTH1 cDNA Transcriptional Unit and Its Expressionin Mammalian Cells[J]. Chinese Journal of Clinical Neurosciences, 1995, 3(4): 191-193 |
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| Authors: | Cui Ning Song Hou-Yan Molecular Genetics Laboratory |
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| Affiliation: | Cui Ning,Song Hou-Yan Molecular Genetics Laboratory .Shanghai Medical University,Shanghai 200032Ying Qi-Long,Jiang Cheng-Chuan,Tang Zhen-Sheng Institute of Neurology,Shanghai Medical University 200040 |
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| Abstract: | The cDNA of human tyrosine hydroxylase type-1(HTH1) was inserted into the EcoR I site of pcDNA3 vector. The recombinant plasmid and pcDNA3 were transfected into COS-7 cells by means of DEAE dextran-mediated method. The HTH1 antigen was found by im-munofluoro-histochemistry stain. The control groups were negative. The result demonstrated that the HTH1 protein could be expressed in mammalian cells containing the expression plasmid of pcDNA3 HTH1. |
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| Keywords: | tyrosine hydroxylase tyrosine hydroxylase cDNA plasmid COS-7 cells transfection |
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