Sensibilisation parentérale du lapin aux protéines du lait de vache : effets sur la perméabilité intestinale

Objectives

Study of the cow's milk protein intolerance mechanisms in order to knowledge, in which could proportions spread the permeability of intestinal to the β-lactoglobulin and α-lactalbumin at the rabbits sensitized to these two proteins.

Methods

The rabbits were sensitized to these proteins by subcutaneous way and their degree of immunization established by Elisa dosage of the specific IgG. For the study of the transepithelial exchanges, the interaction of the proteins (marked to the ¹⁴C-formol), with the mucous intestinal of sensitized and some control rabbits were follow-up in vitro on fragments of ileums mounted in Ussing chamber. Their transepithelial fluxes were measured, after stimulation by the proteins deposed in the mucous compartment from a sampling made from serosa side.

Results

The animals sensitized present some titers in IgG anti β-lactoglobulin and anti α-lactalbumin, as well as some transepithelial fluxes, significantly more increased than control animals.

Conclusion

These results suggests that at the immunized animals, the interaction of the sensitizing protein with intestinal mucous caused some anomalies of hydroelectrolytic transport.