兔膝关节软骨缺损对关节退变的影响

目的:探讨软骨损伤与关节退变之间的关系,以及持续被动运动治疗在其中的作用.方法:新西兰大白兔15只共30个膝关节随机分为3组,采用在关节软骨上钻孔的方法建立软骨损伤模型,术后按照不同的处理分为假手术组、对照组和持续被动运动(CPM)组,各组采取相应的处理方法.术后12周处死实验兔并进行关节软骨大体评分、HE染色评分和MMP-3免疫组化染色.结果:A组软骨表现正常.B组软骨在肉眼观察、病理学表现和MMP-3阳性细胞分数改变上均显示有明显退变.C组的各项指标均与A组接近而与B组差异有显著性意义(P＜0.05).结论:人为造成兔膝关节软骨缺损,可以进展成为骨关节炎(OA).软骨缺损后早期给予CPM治疗8h/d,连续4周,在12周后可有效减缓OA的发生.

Effect of defects in the knee articular cartilage of the rabbit on cartilage degeneration

Objective: To discuss the relationship between cartilage damage and cartilage degeneration and the ef-fect of continuous passive motion on it. Method: 15 New Zealand rabbits with 30 knee cartilage are divided into3 groups randomly. The cartilage damage model was established by drilling on the cartilage. The rabbits that hadbeen operated on were subjected to three forms: group A with fake operation, control group (B) and group C withcontinuous passive motion. Each group has the corresponding post- operative management. The experimental rabbitswere killed at 12th week after operation and were assessed by the gross appearances, hematoxylin and eosion stainand MMP- 3 immunohistochemistry. Result: The cartilage of group A showed normal structure. group B showed ob-viously degeneration which can be observed in gross appearances, the score of pathology and the ration of MMP- 3positive cells. The targets of group C get close to group A. But they have the obvious difference(P<0.05) comparedwith group B. Conclusion:Man- made defects in the knee articular cartilage in rabbits may develop into osteoarthri-tis.The early use of continuous passive motion which was used 8h a day and last 4 weeks after cartilage defectcan avoid the happen of osteoarthritis in 12 weeks.