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| 体外诱导胎盘间充质干细胞向表皮细胞的分化 | |
| 引用本文： | 万振洲,冯亚松,彭海林,张玮,苗宗宁,陈亚宝. 体外诱导胎盘间充质干细胞向表皮细胞的分化[J]. 中国组织工程研究与临床康复, 2011, 0(49): 9223-9226 |
| 作者姓名： | 万振洲 冯亚松 彭海林 张玮 苗宗宁 陈亚宝 |
| 作者单位： | 泰州市第四人民医院检验科;泰州市人民医院检验科;无锡市第三人民医院干细胞实验室 |
| 摘 要： | 背景:以往研究证明胎盘间充质干细胞经诱导可以向骨、软骨、肌肉、脂肪等间充质细胞分化。目的:探讨体外诱导胎盘间充质干细胞分化为表皮细胞的可行性。方法:分离培养胎盘间充质干细胞,流式细胞仪鉴定其表面抗原,用含体积分数为2%胎牛血清以及20μg/L表皮生长因条件培养基诱导,诱导后细胞通过表皮细胞标志物CK19以及CK10染色鉴定。结果与结论:胎盘间充质干细胞表面抗原CD29、CD44、CD105阳性,CD34、CD45、CD106以及HLA-DR阴性。诱后细胞形态明显改变,表皮细胞标志物CK19以及CK10免疫荧光染色阳性,提示胎盘间充质干细胞在体外具有分化为皮细胞的能力,可以作为皮肤组织工程和干细胞研究的一种有效来源。 |
| 关 键 词： | 胎盘 表皮细胞 间充质干细胞 诱导 分化 |
Differentiation of placenta mesenchymal stem cells to epidermal cells in vitro |
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| Wan Zhen-zhou,Feng Ya-song,Peng Hai-lin,Zhang Wei,Miao Zong-ning,Chen Ya-bao. Differentiation of placenta mesenchymal stem cells to epidermal cells in vitro[J]. Journal of Clinical Rehabilitative Tissue Engineering Research, 2011, 0(49): 9223-9226 | |
| Authors: | Wan Zhen-zhou Feng Ya-song Peng Hai-lin Zhang Wei Miao Zong-ning Chen Ya-bao |
| Affiliation: | 1Medical Laboratory of Taizhou Fourth People’s Hospital,Taizhou 225300,Jiangsu Province,China;2Medical Laboratory of Taizhou People’s Hospital,Taizhou 225300,Jiangsu Province,China;3Stem Cell Laboratory of Wuxi Third People’s Hospital,Wuxi 214000,Jiangsu Province,China |
| Abstract: | BACKGROUND:The pervious study has showed that placenta mesenchymal stem cells(PMSCs) can differentiate into bone,cartilage,and muscle and adipose mesenchymal stem cells.OBJECTIVE:To investigate the differentiation of PMSCs into epidermal cells in vitro.METHODS:PMSCs were isolated and cultured and the surface antigen was detected by flow cytometry.RESULTS AND CONCLUSION:Isolated PMSCs were positive for the markers CD105,CD29 and CD44 and negative for typical hematopoietic and endothelial markers CD34,CD45,CD106 and HLA-DR.The morphology of induced cells was obviously changed.Immunohistochemistry stain analysis showed positive for epidermal-specific markers like CK19 and CK10.The PMSCs have the capability to differentiate into epidermal cells in vitro.The placenta may prove to be a useful source of mesenchymal stem cells in skin tissue engineering and stem cells study. |
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