| p53基因药物抑制人鼻咽癌生长的免疫组化研究 |
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| 引用本文： | 敖敏,何刚. p53基因药物抑制人鼻咽癌生长的免疫组化研究[J]. 四川医学, 2009, 30(8): 1193-1194 |
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| 作者姓名： | 敖敏 何刚 |
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| 作者单位： | 四川省人民医院耳鼻咽喉科,四川,成都,610072;四川省人民医院耳鼻咽喉科,四川,成都,610072 |
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| 摘 要： | 目的探索p53基因在鼻咽癌基因治疗方面的可行性。方法以人鼻咽癌CNE细胞株为实验对象,将重组人p53腺病毒药物（10^10rAd／p53）感染人鼻咽癌CNE细胞,用免疫组化实验的方法观察重组人p53腺病毒药物（rAct／p53）对人鼻咽癌CNE细胞体外生长的影响。结果重组人p53腺病毒药物（10^10rAd／p53）对人鼻咽癌CNE细胞生长有明显抑制。结论重组人p53腺病毒药物（rAd／p53）对人鼻咽癌CNE细胞生长能有效抑制,为鼻咽癌的基因治疗提供了实验依据。
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| 关 键 词： | 鼻咽癌 CNE细胞 重组人p53 腺病毒 基因治疗 免疫组化 细胞培养 生长抑制 |
Researeh of gone therapy in human ansopharyngcal cancer with Adenovirus-medinted p53 by immunohistochemistry assay |
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| AO Min,HE Gang. Researeh of gone therapy in human ansopharyngcal cancer with Adenovirus-medinted p53 by immunohistochemistry assay[J]. Sichuan Medical Journal, 2009, 30(8): 1193-1194 |
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| Authors: | AO Min HE Gang |
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| Affiliation: | . (The People＇s Hospital of Sichuan , Chengdu ,Sichuan 610072, China) |
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| Abstract: | Objective To explore the potential use of p53 in gene therapy for human nasopharyngeal cancer. Methods A human nasopharyngeal cancer cell line CNE was used. Adenoviral-mediated transfer of p53 gene（ rAd/p53 ）was transiently in- troduced into CNE cells in vitro. The growth of CNE cells in vitro was examined with immunohistochemistry assay. Results Cell growth was greatly suppressed in vitro. Conclusion rAd/p53 is a potential approach to the gene therapy of nasopharyngeal cancer. |
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| Keywords: | CNE human nasopharyngeal cancer cell line rAd/p53 gene therapy immunohistochemistry assay cell culture growth suppression |
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