| 巨核细胞白血病细胞系HLA-Ⅱ等位基因型的研究 |
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| 引用本文： | 谢桂岚,李志祥.巨核细胞白血病细胞系HLA-Ⅱ等位基因型的研究[J].临床和实验医学杂志,2003,2(2):65-67. |
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| 作者姓名： | 谢桂岚 李志祥 |
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| 作者单位： | 1. 首都医科大学附属北京友谊医院血液内科,北京,100050
2. 首都医科大学附属北京友谊医院移植配型中心,北京,100050 |
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| 摘 要： | 目的 研究巨核细胞(MEG)，白血病细胞系表达人类白细胞抗原ⅡHLA—Ⅱ基因组DNA的等位基因型。提高对该细胞的HLA—Ⅱ基因型的认识。为临床上从分子免疫遗传学水平上探讨巨核细胞白血病发病机理莫定基础。方法 采用分子生物学方法。即：聚合酶链反应—限制性片断长度多态性(PCR—RFLP)技术对MEG白血病细胞系HLA—Ⅱ基因组DNA的等位基因型进行探讨。结果 MEG白血病细胞系的HLA—Ⅱ等位基因型分别是：DRBl：^*1406／^*0410,DQBl：^*0401／^*0401,DPBl：^*0501／^*0501。结论 提高对MEG白血病细胞系HLA—Ⅱ基因型的认识。对今后巨核细胞白血病免疫遗传学的研究具有重要的指导意义。
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| 关 键 词： | 巨核细胞白血病细胞系 HLA－Ⅱ 等位基因 免疫遗传学 聚合酶链反应－限制性片断长度多态性技术 |
| 文章编号： | 1671-4695(2003)02-0065-03 |
Analysis of HLA-Ⅱ allele types of MEG cell lines by PCR-RFLP |
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| XIE Gui - lan,LI Zhi - xiang.Analysis of HLA-Ⅱ allele types of MEG cell lines by PCR-RFLP[J].Journal of Clinical and Experimental Medicine,2003,2(2):65-67. |
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| Authors: | XIE Gui - lan LI Zhi - xiang |
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| Abstract: | Objective The expression of HLA- II genome DNA was investigated in MEG cell lines (human megakaiyoblastic cell lines) . To study the relationship between HLA - II genotype and acute megakaryoblastic leukemia. Methods Polymerase chain reaction - restriction fragment length polymorphism (PCR - RFLP) was used for the investigation of HLA - II genotype of MEG cell line. Results The HLA - II aUeles of MEG cells lines were as Mows: DRB1 * 1406/* 0410, DQB1 * 0401/ * 0401, and DPB1 * 0501/* 0501. Conclusion The study of the expression of HLA - II genotype in MEG cell line is important for understanding im-munogenetics of acute megakaryoblastic leukemia in the clinic. |
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| Keywords: | MEG cell lines HLA- II genotype PCR- RFLP |
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