一测多评法同时测定扶正固本颗粒中6种成分的含量

目的:建立扶正固本颗粒中2,3,5,4′-四羟基二苯乙烯基葡萄糖苷、黄芩苷、淫羊藿苷、汉黄芩苷、黄芩素、汉黄芩素等6种成分含量的测定方法.方法:采用高效液相色谱法.色谱柱为Dikma Diamonsil C18,流动相为乙腈-0.1％磷酸水溶液(梯度洗脱),检测波长为275 nm(0～8 min)、320 nm(8～9...

Simultaneous Determination of the Contents of 6 Components in Fuzheng Guben Granules by QAMS

OBJECTIVE:To establish the method for content determination of 6 components in Fuzheng guben granules,such as 2,3,5,4′-tetrahydroxystilbene glucoside,baicalin,icariin,scutellarin,baicalein and wogonin.METHODS:HPLC method was adopted.The determination was performed on Dikma Diamonsil C18 column with mobile phase consisted of acetonitrile-0.1%phosphoric acid aqueous solution(gradient elution)at the flow rate of 1.0 m L/min.The detection wavelengths were set at 275 nm(0-8 min),320 nm(8-9 min)and 275 nm(9-33 min).The column temperature was set at 25℃,and sample size was 10μL.With baicalin as reference material,the relative correction factors(fk/s)of other five components were calculated by multi-point correction method and slope correction method;the retention time difference method was used to locate the chromatographic peaks;the calculation values obtained by above 2 QAMS were compared with measured values of external standard method.RESULTS:The linear range of 2,3,5,4′-tetrahydroxystilbene glucoside,baicalin,icariin,scutellarin,baicalein and wogonin were 0.053-2.12,0.163-6.52,0.059-2.36,0.0216-0.864,0.03-1.2,0.021-0.84μg(r>0.999),respectively.RSDs of precision,stability(12 h)and reproducibility tests were all lower than 3%.Average recoveries were 98.72%-99.82%(RSDs were 0.89%-1.24%,n=9).Using baicalin as reference material,fk/s of multi-point correction method for 2,3,5,4′-tetrahydroxystilbene glucoside,icariin,scutellarin,baicalein and wogonin were 1.172,0.528,1.479,1.820 and 2.534,respectively;fk/sof slope correction method were 1.234,0.550,1.559,1.939,2.664.RSDs of 6 components in 10 batches of Fuzheng guben granules by 3 methods were 0.29%-2.77%(n=10),respectively.Pearson correlation coefficient was not lower than 0.9999(P<0.001)in measured values between QAMS and external standard method.CONCLUSIONS:QAMS method is established successfully for simultaneous determination of 6 components in Fuzheng guben granules.