体外联合培养条件下小鼠睾丸足细胞诱导成熟T淋巴细胞凋亡

目的 :探讨体外联合培养条件下睾丸足细胞能否诱导 T淋巴细胞凋亡。方法 :用 TU NEL 标记、核酸电泳、电镜、流式细胞术检测对照组 (T淋巴细胞 )、A组 (T淋巴细胞 +培养睾丸足细胞 2 4h培养基上清 )、B组 (T淋巴细胞 +睾丸足细胞 )等 3组中小鼠成熟 T淋巴细胞的凋亡及其数量的变化。结果 :3组 T淋巴细胞在电镜下均可见细胞染色质浓集 ,核固缩裂解 ,凋亡小体形成 ;TUNEL 标记见部分细胞核染色呈深蓝色 ;核酸电泳出现典型 DNA梯状带 ;流式细胞术检测 A,B两组 T淋巴细胞凋亡量均明显高于对照组 (P<0 .0 1) ,B组 T淋巴细胞凋亡数量显著高于 A组 (P<0 .0 1)。 结论 :体外培养条件下小鼠睾丸足细胞能够诱导成熟 T淋巴细胞凋亡。

Mouse sertoli cells induced mature T lymphocytes apoptosis in cocultures

Objective:To study whether mouse sertoli cells can induce the apoptosis of matured T lymphocytes in cocultures. Methods: With TUNEL, DNA electrophoresis, electromicrography and flow cytometry, we examined the apoptosis of mouse matured T lymphocytes in control group (T lymphocytes only), group A (T lymphocytes + the 24 h cultured medium of sertoli cells), group B (T lymphocytes+sertoli cells). Results: Under electromicrography, chromatin condensation, nucleus shrinkage and apoptotic body were observed in T lymphocytes from 3 groups; some nucleus were stained dark blue with TUNEL; a typical DNA ladder was found with DNA electrophoresis. The apoptotic rates of T lymphocytes in group A and B were significantly higher than that in control group ( P <0.01). The apoptotic rate of T lymphocytes in group A was significantly lower than that in group B ( P <0.01). Conclusion: In coculture condition in vitro , mouse sertoli cells can induce the apoptosis of matured T lymphocytes.