HPLC法测定环磷腺苷葡胺注射液中环磷腺苷的含量

目的：建立HPLC法测定环磷腺苷葡胺注射液中环磷腺苷的含量。方法：采用HypersilC18柱（250mm×4．6mm，5μm），流动相为0．01％磷酸溶液-乙腈（95：5），流速为1．0ml·min^-1，检测波长为259nm，柱温为30℃。结果：环磷腺苷在0．051-2．054μg范围内线性关系良好，r=1．0000（n=6），平均回收率为99．61％（n=9），RSD为0．80％。结论：该方法灵敏、准确、专属性强，能简便有效控制环磷腺苷葡胺注射液中环磷腺苷的含量。

Determination of cAMP in Meglumine Adenosine Cyclophosphate Injection by HPLC

Objective： To establish an HPLC method for the determination of cAMP in meglumine adenosine cyciophosphate injection. Method： Hypersil C18 column （250 mm ×4. 6 mm, 5μm） set at 30 ℃ was used . The mobile phase was 0. 01% H3PO4 solution and acetonitrile （95：5） whb flow rate of 1.0 ml·min ^-1, and the detection wavelength was set at 259 nm. Result： A good linearity was obtained over the range of 0. 051-2. 054 lag（r = 1. 000 0, n = 6）. The average recovery was 99. 61% （RSD = 0. 80%, n = 9）. Conclusion： The method is sensitive, accurate and specific,and can be used for the quality eontrol of meglumine adenosine cyelophosphate injection.