慢性丙型肝炎肝组织CPP32、Fas和Fas配体的表达及其与病毒抗原的关系

目的 探讨慢性丙型病毒性肝炎（ＣＨＣ）中Ｆａｓ－ＦａｓＬ－ＣＰＰ３２介导的细胞凋亡的意义及其与丙型肝炎病毒（ＨＣＶ）抗原表达间的关系。方法 用免疫组织化学和核酸原位杂交法，在连续组织切片上对６５例不同病变程度的ＣＨＣ穿刺活检肝组织中ＣＰＰ３２、Ｆａｓ和ＦａｓＬ蛋白（酶）及其ｍＲＮＡ的表达进行检测。同时用免疫组织化学方法检测ＨＣＶ核心抗原、ＮＳ３和ＮＳ５抗原的表达，原位检测结果用图象分析作定量测定，

Expression of CPP32, Fas and Fas ligand and the relationship between their expression and viral antigens in chronic hepatitis C

Objective To study the significance of Fas-Fas ligand (FasL)-CPP32 mediated hepatocytic apoptosis and the relationship between their expression and hepatitis C virus (HCV) antigens in pathogenesis of chronic hepatitis C (CHC). Methods The expression of Fas and FasL proteins and CPP32 protease and their mRNAs was detected by immunohistochemistry and in situ hybridization respectively. Meanwhile, HCV core protein and nonstructural protein NS_3 and NS_5 were examined on the sequential liver tissue sections from 65 cases of patients with CHC and 5 cases of normal subjects. The positive staining was quantified by image analysis. Results in CHC liver tissues, CPP32 protease, Fas and their mRNAs were mainly expressed in the hepatocytes, especially in the HCV core protein-positive hepatocytes and their surroundings, but FasL protein and its mRNA were expressed dominantly in the infiltrating mononuclear cells in the portal area and hepatic sinusoid. According to the increase of histological activity index (HAI) in the CHC liver tissues, the expression of the three proteins (protease) and their mRNAs were upregrulated obviously. Conclusion The results suggest i) that Fas-FasL-CPP32 system is associated with HAI of CHC, ii) that HCV core antigen may affect the expression of CPP32 and Fas hepatocytes apoptosis.