间接共培养诱导大鼠骨髓间充质干细胞向牙周膜成纤维样细胞分化

目的：探讨通过间接共培养诱导骨髓间充质干细胞（Bone marrow mesenchymal stem cells,BMSCs）向牙周膜成纤维细胞（Periodontal ligament fibroblasts,PDLFs）分化的可行性。方法：将大鼠BMSCs与PDLFs间接共培养,分别于不同时间段检测碱性磷酸酶（Alkaline phosphatase,ALP）活性以及骨钙素（Osteocalcin,OCN）、Ⅲ型胶原（Collagen typeIII,COLⅢ）mRNA表达的变化。结果：间接共培养后的BMSCs表现出类似PDLFs的性质,各检测指标与单独培养的PDLFs无统计学差异,与单独培养的BMSCs有统计学差异。结论：牙周膜成纤维细胞通过旁分泌机制可以诱导骨髓间充质干细胞向其分化。

Induction effect on the differentiation of rat bone marrow mesenchymal stem cells Co-cultured with periodontal ligament fibroblasts

Objective：To investigate the feasibility of inducing the differentiation of rat bone marrow mesenchymal stem cells（BMSCs）under the influence of periodontal ligament fibroblasts（PDLFs）in vitro. Method：The non-contact co-culture system was established by cell culture inserts. The rat BMSCs were co-cultured with the rat PDLFs for 14 days. The expression of PDLFs protein markers（ALP,OCN,COL Ⅲ）were measured at different time courses. Result：The rat bone mesenchymal stem cells acquire the characteristics of the periodontal ligament fibroblasts .There are no differences of all markers between co-cultured BMSCs and PDLFs cultured individually,and observed changes between co-cultured BMSCs and BM-SCs cultured individually. Conclusion：The factors secreted by PDLFs may induce the rat BMSCs to obtain periodontal ligament-like marker expression.