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Comparison of the lignin-degrading white rot fungi Phanerochaete chrysosporium and Sporotrichum pulverulentum at the DNA level
Authors:Ute Raeder  Paul Broda
Affiliation:(1) Department of Biochemistry and Applied Molecular Biology, University of Manchester Institute of Science and Technology, P. 0. Box 88, M60 1QD Manchester, UK
Abstract:
Summary DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium ME446 and S. pulverulentum Novobranova both have a GC-content of 59% for chromosomal DNA with the rRNA genes present as an AT-rich satellite; the mitochondrial DNA has a GC-content of 33%. The genome size estimated for P. chrysosporium ME446 is about 4–5 × 107 bp.
Keywords:Lignin degradation  Phanerochaete chrysosporium  DNA homology
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