| 神经生长因子促进海马胆碱能神经再生过程中Lhx8的表达 |
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| 引用本文： | 李浩明,朱培培,金国华,施金洪,邹琳清,田美玲,衣昕,秦建兵. 神经生长因子促进海马胆碱能神经再生过程中Lhx8的表达[J]. 解剖学报, 2014, 0(4): 441-445. DOI: 10.3969/j.issn.0529-1356.2014.04.001 |
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| 作者姓名： | 李浩明 朱培培 金国华 施金洪 邹琳清 田美玲 衣昕 秦建兵 |
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| 作者单位： | 南通大学医学院人体解剖学系 江苏省神经再生重点实验室，江苏 南通 226001 |
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| 基金项目： | 国家自然科学基金资助项目;江苏高校优势学科建设工程资助项目 |
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| 摘 要： | 目的 探讨神经生长因子（NGF）促进海马胆碱能神经再生与同源盒基因Lhx8之间的关系。方法 SD大鼠72只，在制备切割右侧穹隆海马伞模型和NGF侧脑室注射的基础上，分为对照组、切割组、NGF组、切割联合NGF组；Real-time PCR和Western blotting分别检测各组动物海马中Lhx8蛋白的表达变化；免疫荧光技术检测各组动物海马齿状回颗粒下层中胆碱乙酰转移酶(ChAT)/Lhx8双标细胞数。 结果 切割组和NGF组中Lhx8蛋白的表达量较对照组增高，切割联合NGF组增高更为明显，且Lhx8基因表达也增高；NGF组和切割联合NGF组中海马齿状回颗粒下层(SGZ)中的ChAT/Lhx8双标细胞数也较对照组和切割组明显增多。结论 侧脑室注射NGF促进海马胆碱能神经再生与Lhx8的高表达有关。
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| 关 键 词： | Lhx8 海马 神经生长因子 胆碱能神经 再生 实时定量聚合酶链反应 大鼠 |
| 收稿时间： | 2013-07-18 |
Dynamic expression of Lhx8 in nerve growth factor induced hippocampal neuroregeneration |
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| LI Hao-ming ZHU Pei-pei JIN Guo-hua SHI Jin-hong ZOU Lin-qing TIAN Mei-ling YI Xin QIN Jian-bing. Dynamic expression of Lhx8 in nerve growth factor induced hippocampal neuroregeneration[J]. Acta Anatomica Sinica, 2014, 0(4): 441-445. DOI: 10.3969/j.issn.0529-1356.2014.04.001 |
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| Authors: | LI Hao-ming ZHU Pei-pei JIN Guo-hua SHI Jin-hong ZOU Lin-qing TIAN Mei-ling YI Xin QIN Jian-bing |
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| Affiliation: | Department of Anatomy, Medical School, Jiangsu Key Laboratory of Neuroregeneration, Nantong University, Jiangsu Nantong 226001, China |
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| Abstract: | Objective To investigate the relationship between the nerve growth factor (NGF) induced hippocampal neuroregeneration and homeobox gene Lhx8.Methods Seventy-two SD rats were divided into control group, transected group, NGF group, transected combined with NGF group after right fimbria-fornix transection and NGF intracerebroventricular injection. Real-time PCR and Western blotting were applied to detect the gene and protein expression of Lhx8 in each group. The choline acetyltransferase (ChAT)/Lhx8 double labeled cells in subgranular zone (SGZ) of hippocampus in each group were detected by immunofluorescence. Results The expression of Lhx8 gene and protein in the transected, NGF group and especially in the transected combined with NGF group was obviously higher than in the control group. The number of ChAT/Lhx8 double labeled cells in the NGF group and the transected combined with NGF group was obviously more than in the control group and transected group. Conclusion The hippocampal neuroregeneration which induced by NGF intracerebroventricular injection was associated with the higher expression of Lhx8. |
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| Keywords: | Lhx8 Hippocampus Nerve growth factor Cholinergic nerve Regeneration Real-time PCR Rat |
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