电针对失血性低血压家兔心肌细胞游离钙镁浓度的影响

用ＡＲ－ＣＭ－ＣＯＭ阳离子测定系统和离子探针Ｆｕｒａ－２－ＡＭ及Ｆｕｒａｐｔｒａ－ＡＭ,观察了电针在治疗家兔实验性低血压时心肌细胞内游离钙离子浓度（［Ｃａ~（２＋）］ｉ）和游离镁离子浓度（［Ｍｇ~（２＋）］ｉ）的变化情况。股动脉放血造成家免失血性低血压,心肌细胞［Ｃａ~（２＋）］ｉ随血压降低而降低,［Ｍｇ~（２＋）］ｉ则升高,［ Ｃａ~（２＋）］ ｉ／［ Ｍｇ~（２＋）］ ｉ比值减小;电针拟“人中”、“承浆”穴位后,治疗组家兔血压及［ Ｃａ~（２＋）］ ｉ均明显高于低血压对照组,［Ｃａ~（２＋）］ｉ／［ Ｍｇ~（２＋）］ ｉ比值相应增大;电针对正常家兔心肌细胞［Ｃａ~（２＋）］ｉ和［Ｍｇ~（２＋）］ｉ均无明显影响。结果提示心肌细胞内游离Ｃａ~（２＋）、Ｍｇ~（２＋）在针刺调整血压的过程中起着一定的作用。

Effects of Acupuncture on Intracellular Free Calcium and Magnesium Concentrations in Cardiac Myocytes of Hemorrhagic Hypotension Rabbits

The effects of electroacupuncture on hypotension and the concentrations of intracellular free calcium ( Ca~(2+)]i) and free magnesium ( Mg~(2+) ] i) in cardiac myocyte of rabbit were studied. The hemorrhagic hypotension was induced by losing blood from femoral artery of rabbit. Ca~(2+) ]i and Mg~(2+) ]i were determined by use of AR-CM-COM canon measurement system and the ionic probe fura-2 and fu- raptra. Ca~(2+)] i was significantly decreased, while Mg~(2+) ] i was increased, and the ratio of Ca~(2+) ] i/ Mg~(2+) ] i was decreased as the blood pressure went down with losing blood. The blood pressure and Ca~(2+) ] i of treatment group rabbits were significantly raised by electroacupuncturing the points of " Renzhong" and "Chengjiang", and the ratio of Ca~(2 +) ] i/ Mg~(2+)] i was increased. The cardiac myocyte Ca~(2+)] i and Mg~(2+) ] i of normal rabbits had no clear changes by electroacupuncture. It suggested that intracellular free calcium and magnesium ion in cardiac myocyte may play an important role in regulating blood pressure by acupuncture.