COX-2、PCNA及p53在鼻咽癌中的表达及意义

目的:研究COX-2、PCNA及p53的基因产物在鼻咽癌组织中的表达及与其生物学行为和生存率之间的关系。方法:采用免疫组化SP法检测60例鼻咽癌及30例正常鼻咽组织中COX-2、PCNA及p53的蛋白表达水平。结果:(1)鼻咽癌组织COX-2、PCNA及p53阳性表达率分别为90%、75%和68%,明显高于正常鼻咽组织;(2)鼻咽癌组织COX-2、PCNA及p53的表达与临床分期、分化程度和淋巴结转移相关(P<0.05);(3)COX-2、PCNA及p53的表达密切相关;(4)鼻咽癌组织COX-2、PCNA阳性表达和生存率呈负相关,是鼻咽癌复发的显著预后因素。结论:鼻咽癌组织中COX-2、PCNA表达水平明显上调,与鼻咽癌的发生发展及预后不良有关,免疫组化检测COX-2、PCNA表达可用以判断鼻咽癌患者的预后。

Prognostic significance of COX-2,PCNA and p53 in nasopharyngeal carcinoma

Objective: To ascertain the possible roles of COX-2,PCNA and p53 in the tumorigenesis and development of nasopharyngeal carcinoma,and to evalulate their impact on the prognosis of nasopharyngeal carcinoma.Methods: Immunohistochemical assay was used to determine COX-2,PCNA and p53 in all specimens,including 60 cases of nasopharyngeal carcinoma and 30 cases of normal nasopharynx.Results:(1) The overall positive expression rate of COX-2,PCNA and p53 in nasopharyngeal carcinoma was 90%,75% and 68% respectively,higher than that in the normal nasopharynx.(2) Intensity of expression of COX-2,PCNA and p53 in nasopharyngeal carcinoma was related to tumor stage,tumor differentiation and lymph node metastasis(P<0.05).(3) Expressions of COX-2,PCNA and p53 were correlated with each other in nasopharyngeal carcinoma.(4) Positive COX-2 and PCNA were associated with a lower 5-year survival and they were significant prognostic variables for recurrence(P<0.05).Conclusion: COX-2,PCNA and p53 may play vital roles in the tumorigenesis and development of the cancer via different methods.Unfavorable prognosis was associated with expression of COX-2 and PCNA while not with p53.Immunohistochemical assessment of COX-2 and PCNA might be a useful molecular marker for predicting prognosis in nasopharyngeal cancer.