| 高效液相色谱测定大鼠心肌组织腺苷酸含量 |
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| 引用本文： | 缪宇,王承龙,殷惠军,史大卓,陈可冀. 高效液相色谱测定大鼠心肌组织腺苷酸含量[J]. 北京大学学报(医学版), 2005, 37(2): 201-202 |
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| 作者姓名： | 缪宇 王承龙 殷惠军 史大卓 陈可冀 |
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| 作者单位： | 中国中医研究院西苑医院心内科,北京,100091;中国中医研究院西苑医院心内科,北京,100091;中国中医研究院西苑医院心内科,北京,100091;中国中医研究院西苑医院心内科,北京,100091;中国中医研究院西苑医院心内科,北京,100091 |
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| 摘 要： | 目的:优化高效液相色谱(HPLC)法检测大鼠心肌组织腺苷酸的含量.方法:ODS HYPERSIL C18分析柱,检测波长254 nm,流动相为50 mmol/L磷酸钾缓冲液(pH 6.5).结果:心肌三磷酸腺苷(ATP)、二磷酸腺苷(ADP)和一磷酸腺苷(AMP)平均回收率分别为99%～107%,96%～104%和95%～119%;日内和日间相对标准偏差(RSD)分别在1.5%和5.1%以下.结论:优化高效液相色谱法快速准确、简便易行.
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| 关 键 词： | 色谱法 高压液相 心肌 腺苷酸 |
| 文章编号： | 1671-167X(2005)02-0201-02 |
| 修稿时间： | 2004-09-13 |
High performance liquid chromatogram (HPLC) determination of adenosine phosphates in rat myocardium |
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| MIAO Yu,WANG Cheng-long,Yin Hui-jun,SHI Da-zhuo,CHEN Ke-ji. High performance liquid chromatogram (HPLC) determination of adenosine phosphates in rat myocardium[J]. Journal of Peking University. Health sciences, 2005, 37(2): 201-202 |
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| Authors: | MIAO Yu WANG Cheng-long Yin Hui-jun SHI Da-zhuo CHEN Ke-ji |
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| Affiliation: | Department of Cardiology, Xi yuan Hospital, China Academy of Traditional Chinese Medicine, Beijing 100091, China. miaoyu0609@sina.com |
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| Abstract: | Objective: To establish method for the quantitative determination of adenosine phosphates in rat myocardium by optimized high performance liquid chromatogram (HPLC). Methods: ODS HYPERSIL C 18 column and a mobile phase of 50 mmol/L tribasic potassium phosphate buffer solution (pH 6.5), with UV detector at 254 nm were used. Results: The average recovery rates of myocardial adenosine triphosphate (ATP), adenosine diphosphate(ADP)and adenosine monophosphate (AMP) were 99%-107%, 96%-104% and 95%-119%, respectively;relative standard deviations(RSDs)of within-day and between-days were less than 1.5% and 5.1%, respectively. Conclusion: The method is simple, rapid and accurate, and can be used to analyse the adenosine phosphates in myocardium. |
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| Keywords: | Chromatography high pressure liquid Myocardium Adenosine phosphates |
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