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| 阿尔茨海默病大鼠海马热休克蛋白70表达变化的研究 | |
| 引用本文： | 鲍娟,杨期东,罗红波,苗旺,郭伟新. 阿尔茨海默病大鼠海马热休克蛋白70表达变化的研究[J]. 脑与神经疾病杂志, 2008, 16(5) |
| 作者姓名： | 鲍娟 杨期东 罗红波 苗旺 郭伟新 |
| 作者单位： | 长沙中南大学湘雅医院神经内科,湖南,410008;长沙中南大学湘雅医院神经内科,湖南,410008;长沙中南大学湘雅医院神经内科,湖南,410008;长沙中南大学湘雅医院神经内科,湖南,410008;长沙中南大学湘雅医院神经内科,湖南,410008 |
| 摘 要： | 目的:双侧海马注射Aβ1-42建立大鼠阿尔茨海默病(AD)模型,研究其海马热休克蛋白70(HSP70)表达的变化并探讨与AD病理进程可能存在的联系。方法:48只SD大鼠随机分为模型组与生理盐水对照组,双侧海马分别注射Aβ1-42,分别于术后1天、7天、14天、21天在Y迷宫行为学测试后处死大鼠,采用RT-PCR和Western-blot方法,观察各组大鼠海马HSP70表达的变化。结果:术后14天、21天模型组学习记忆能力较对照组和术前明显减退(P<0.05)。术后模型组大鼠海马HSP70表达逐渐减少,术后7天(mRNA:0.34±0.05;蛋白:0.29±0.03)、14天(mRNA:0.32±0.06;蛋白:0.23±0.04)与对照组比较差异有统计学意义(P<0.05),至术后21天,模型组HSP70表达减少最为明显(mRNA:0.29±0.04;蛋白:0.11±0.03)(P<0.01)。结论:大鼠海马注射Aβ1-42导致HSP70表达减少,HSP70表达减少可能参与了AD的病理发展过程。 |
| 关 键 词： | 阿尔茨海默病 热休克蛋白70 |
The expressing changes of heat shock protein 70 in hippocampus of rat with Alzheimer's disease |
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| BAO Juan,YANG Qi-dong,LUO Hong-bo,et al.. The expressing changes of heat shock protein 70 in hippocampus of rat with Alzheimer's disease[J]. Journal of Brain and Nervous Diseases, 2008, 16(5) | |
| Authors: | BAO Juan YANG Qi-dong LUO Hong-bo et al. |
| Affiliation: | BAO Juan,YANG Qi-dong,LUO Hong-bo,et al.Department of Neurology,Xiangya Hospital of Central South University,Changsha 410008,China |
| Abstract: | Objective :To study the expressing changes of heat shock protein 70(HSP70)in hippocampus of rats with Alzheimer s disease(AD)induced by Aβ1-42 administrated into hippocampus.Methods :48 healthy SD rats were randomly divided into model group injected with Aβ1-42 and control group injected with normal saline into hippocampus.Y maze test was used to study the learning and memory ability of the rats at the 7th,14th and 21st day after injection into hippocampus.HSP70 expressions in hippocampus tissues were detec... |
| Keywords: | Alzheimer disease heat shock protein 70 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |