2型糖尿病小鼠海马锥体细胞及毛细血管电镜观察

目的 研究2型糖尿病模型db/db小鼠海马锥体细胞、毛细血管超微结构变化。方法 糖尿病组：6周龄C57BL／KsJ(db db )小鼠5只。对照组：非糖尿病小鼠C57BL／KsJ(?/ )5只。于30周龄时,灌注固定取脑、透射电镜下观察海马CA1区。结果 电镜下可见糖尿病小鼠海马锥体细胞凋亡、毛细血管基底膜增厚。结论 如上改变可能是糖尿病认知功能 减退的病理基础。

The study of the hippocampus pyramidal cells and capillaries in type 2 diabetic mice under electron microscope

Objective To study the ultrastructure changes of hippocampus pyramidal cells and capillaries in genetically diabetic mice C57BL/KsJ (db/db).Methods We chose 5 obese C57BL/KsJ db/db mice of 6 weeks old with fast blood glucose (FBG) higher than 11 1mmol/L as diabetic group and 5 normal weight C57BL/KsJ (?/ ) mice with FBG lower than 6 0mmol/L as normal group. Mice were killed at 30 weeks and hippocampus samples were embedded in Epon 812. Thin sections were cut with ultrathin microtome and observed with electron microscopes. Results Pyramidal cells of hippocampus in diabetic mice had significant retrograde changes. The basement membrane of capillaries thickened significantly and endothelial cells and pericytes degenerated.Conclusion The significant pathological changes in hippocampus pyramidal cells and capillaries of diabetic mice may related to dysfunction of cognition.