| 正交试验优化文蛤水溶性多糖的水提醇沉工艺 |
| |
| 引用本文： | 陈丽叶,王令充,吴皓,程建明,刘睿,柴尧,彭九嫚,冯子芳,程颖. 正交试验优化文蛤水溶性多糖的水提醇沉工艺[J]. 中国实验方剂学杂志, 2016, 22(5): 18-21 |
| |
| 作者姓名： | 陈丽叶 王令充 吴皓 程建明 刘睿 柴尧 彭九嫚 冯子芳 程颖 |
| |
| 作者单位： | 南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023,南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023,南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023,南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023,南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023,南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023,南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023,南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023,南京中医药大学 江苏省海洋药用生物资源研究与开发重点实验室, 江苏省中药资源产业化过程协同创新中心, 南京 210023 |
| |
| 基金项目： | 国家公益性行业专项(201305007,201405017);国家高新技术研究发展计划(863计划)项目(2013AA093003);江苏高校优势学科建设项目(PAPD) |
| |
| 摘 要： | 目的:优选文蛤水溶性多糖的水提醇沉工艺,为该有效部位的保健品开发提供实验依据。方法:采用蒽酮-硫酸法测定水溶性多糖的含量,检测波长625 nm。以水溶性多糖转移率为指标,通过单因素试验考察提取溶剂p H及绞碎程度对文蛤水溶性多糖转移率的影响。以水溶性多糖转移率为指标,通过正交试验考察加水量、提取时间、提取次数对文蛤水溶性多糖水提工艺的影响。以水溶性多糖得率及质量分数的综合评分为指标,通过正交试验考察浓缩程度、醇沉浓度、醇沉时间对文蛤水溶性多糖醇沉工艺的影响。结果:文蛤水溶性多糖的最佳水提工艺为加3倍水煎煮3次,每次40 min,水提液浓缩至原料的1/2,加乙醇醇沉至体积分数达80%,醇沉12 h,抽滤,60℃减压干燥。文蛤水溶性多糖得率7.71%,质量分数42.53%。结论:优选的文蛤水溶性多糖水提醇沉工艺稳定可行,可兼顾该有效部位的得率和纯度,为该部位的研究与开发提供参考。
|
| 关 键 词： | 文蛤 总多糖 蒽酮-硫酸法 转移率 |
| 收稿时间： | 2015-07-08 |
Optimization of Extraction Technology and Alcohol Precipitation Process for Water-soluble Polysaccharides in Meretrix meretrix by Orthogonal Test |
| |
| CHEN Li-ye,WANG Ling-chong,WU Hao,CHENG Jian-ming,LIU Rui,CHAI Yao,PENG Jiu-man,FENG Zi-fang and CHENG Ying. Optimization of Extraction Technology and Alcohol Precipitation Process for Water-soluble Polysaccharides in Meretrix meretrix by Orthogonal Test[J]. China Journal of Experimental Traditional Medical Formulae, 2016, 22(5): 18-21 |
| |
| Authors: | CHEN Li-ye WANG Ling-chong WU Hao CHENG Jian-ming LIU Rui CHAI Yao PENG Jiu-man FENG Zi-fang CHENG Ying |
| |
| Affiliation: | Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China,Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China and Jiangsu Collaborative Innovation Center of Chinese Medicinal Resources Industrialization, Jiangsu Key Laboratory of Research and Development in Marine Bio-resource Pharmaceutics, Nanjing University of Chinese Medicine, Nanjing 210023, China |
| |
| Abstract: | |
| |
| Keywords: | Meretrix meretrix total polysaccharides anthrone-sulfuric acid method transfer rate |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《中国实验方剂学杂志》浏览原始摘要信息 |
|
点击此处可从《中国实验方剂学杂志》下载全文 |
|
