不同产地姜黄中挥发油及姜黄色素的含量分析

目的:明确姜黄有效成分在不同地区的含量差异,考察姜黄素、去甲氧基姜黄素、双去甲氧基姜黄素与挥发油之间含量的相关关系。方法:采集不同产地姜黄样品,结合HPLC-UV及2010年版《中国药典》中挥发油测定法分别分析姜黄中姜黄色素和挥发油的含量,采用SPSS 20.0软件的聚类分析及相关分析功能对测定的含量进行分析。结果:不同产地姜黄有效成分含量差异明显,姜黄色素质量分数最大值约4.9%,最小值仅约0.2%,相差约24.5倍;姜黄挥发油最大值与最小值之间差距达4.2倍。姜黄的传统道地产区含量优势明显,不同姜黄色素与挥发油之间均呈现极显著正相关关系。结论:海拔差异对姜黄中有效成分的含量变化有一定影响,姜黄的产区需要进行合理的规划以提高其品质并推进其产业的可持续发展。

Quantitative Analysis of Volatile Oil and Curcuminoids in Curcumae Longae Rhizoma from Different Areas

Objective: To investigate quality of Curcumae Longae Rhizoma from different areas by analyzing correlation relationship of demethoxycurcumin,bisdemethoxycurcumin,curcumin and volatile oil in this herb. Method: Samples of Curcumae Longae Rhizomafrom different areas were collected,contents of curcuminoids were quantified by HPLC-UV whereas volatile oil was determinate by this method in the 2010 edition of Chinese Pharmacopoeia.Determination were analyzed by cluster and person correlation analysis with SPSS 20.0 software. Result: Effective ingredientsin Curcumae Longae Rhizomafrom different areas had a remarkable difference,when a maximum value of curcuminoids was about 4.9%,a minimum value was about 0.2%.Content of volatile oil in Curcumae Longae Rhizoma between the maximum and minimum values reached 4.2 times.Significant positive correlation had been showed between demethoxycurcumin,bisdemethoxycurcumin,curcumin and volatile oil. Conclusion: Altitude difference has effecton contents of curcuminoids in Curcumae Longae Rhizoma.Farming location of Curcumae Longae Rhizoma need to carry on reasonable planning to improve its quality and to promote sustainable development of industry.