| 非酒精性脂肪性肝炎大鼠肝组织Bcl-2和Bax的表达及其意义 |
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| 引用本文： | 解燕茹,赵和平.非酒精性脂肪性肝炎大鼠肝组织Bcl-2和Bax的表达及其意义[J].实用肝脏病杂志,2009,12(6):411-413. |
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| 作者姓名： | 解燕茹 赵和平 |
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| 作者单位： | 1. 山西医科大学,太原市,030001
2. 第一临床医院综合科 |
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| 摘 要： | 目的探讨凋亡调控蛋白Bel-2和Bax表达在大鼠非酒精性脂肪性肝炎发病中的作用。方法将20只Wistar大鼠随机分为普通饲料喂养组（正常组）和高脂饲料喂养组（模型组），连续喂养12周后，测定血清甘油三酯（TG）、总胆固醇（TC）、丙氨酸氨基转移酶（ALT）、天冬氨酸氨基转移酶（AST），观察肝组织病理学变化和炎症活动度计分，采用westem blot法检测肝组织Bcl-2和Bax的表达。结果模型组大鼠肝小叶内肝细胞呈弥漫性脂肪变，伴大量炎性细胞浸润及散在性点状坏死；模型组动物肝组织Bax蛋白表达较正常组显著性升高（1．02±0．33对0．51±0．03，P〈0．01），而Bel-2蛋白较正常组则明显下降（0．15±0．31对0．52±0．01，P〈0．01），Bel-2／Bax比值降低（0．14±0．01对1．03±0．06，P〈0．01）。结论凋亡调控蛋白Bel-2和Bax的表达变化可能参与了NASH的发生和发展过程。
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| 关 键 词： | 非酒精性脂肪性肝炎 细胞凋亡 Bcl-2 Bax |
Expressions of Bcl-2 and Bax in liver tissures of rats with nonalcoholic steatohepatits |
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| Institution: | XIE Yanru,ZHAO Hep-ing.( Department of Synthetical Section,Affiliated Hospital,Shanxi Medical University, Taiyuan,Shanxi 030001,China) |
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| Abstract: | Objective To explore the effect of apoptosis-regulated proteins Bel-2 and Bax on the liver of rats with nonalcoholic steatohepatitis. Methords 20 male Wistar rats were randomly divided into standard diet-fed group and high fat diet-fed group. After 12 weeks,the levels of serum triglyceride,total cholesterol,ALT and AST were measured. The levels of Bcl-2 and Bax Proteins were determined by Western blot. Results The NASH rats exhibited severe steatohepatitis with scattered punctual necrosis;The relative level of Bax in liver tissue of rats with NASH was significantly higher than that in controls（1.02±0.33 vs.0.51±0.03,P〈0.01）,while the expression of Bcl-2 was decreased（0.15±0.31 vs 0.52±0.01,P〈0.01）. The Bcl-2/Bax ratio also decreased in the NASH group（0.14±0.01 vs 1.03±0.06,P〈0.01）. Conclusion Bcl-2 and Bax proteins could play a role in rats with nonalcoholic steatohepatitis. |
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| Keywords: | Bcl-2 Bax |
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