逍遥散加减方对高催乳素血症大鼠卵巢自分泌及旁分泌机制的影响

目的:探讨逍遥散加减方对高催乳素血症大鼠模型卵巢自分泌及旁分泌机制的影响。方法:采用腹腔注射甲氧氯普胺制成高催乳血症大鼠模型,随机分为6组:空白组、模型组、逍遥散加减方高、中、低剂量组(分别予以逍遥散加减方60、30、15g·kg^-1·d^-1)、溴隐亭组0.001g·kg^-1·d^-1,药物治疗30d后通过免疫组化检测大鼠卵巢组织胰岛素样生长因子1(IGF-1)、胰岛素样生长因子2(IGF-2)阳性结果的表达;采用MTT检测卵巢颗粒细胞存活率及增殖率;采用ELISA法测定卵巢抗苗勒试管激素(AMH)的含量;采用PCR检测血清促卵泡激素受体(FSHR)的含量。结果:与空白组比较,模型组IGF-1、AMH、FSHR、卵巢颗粒细胞存活率、细胞增殖显著减少(P<0.05,P<0.01);逍遥散加减方中、低剂量组IGF-1显著降低(P<0.01),逍遥散加减方低剂量组AMH及卵巢颗粒细胞存活率显著降低(P<0.05),溴隐亭组IGF-1、卵巢颗粒细胞存活率显著降低(P<0.05);与模型组比较,逍遥散加减方高剂量组AMH、IGF-1、卵巢颗粒细胞存活率显著升高(P<0.01,P<0.05),逍遥散加减方高剂量组细胞增殖率及FSHR升高(P<0.05,P<0.01)。结论:逍遥散加减方高剂量组通过提高IGF-1、FSHR及AMH的含量调节卵巢自分泌/旁分泌机制,进而提高动物模型卵巢颗粒细胞存活率。

Effects of Modified Xiaoyao Powder on ovary of hyperprolactinemia rats of autocrine and paracrine mechanisms

Objective:To investigate the effects of Modified Xiaoyao Powder on ovarian autocrine and paracrine in rats with hyperprolactinemia.Methods:Rat model of hyperprolactinemia was made by intraperitoneal injection of metoclopramide.The rats were randomly divided into 6 groups:blank group,model group,high,middle and low dose groups of Modified Xiaoyao Powder(60,30,15 g·kg^-1·d^-1,respectively),bromocriptine group 0.001 g·kg^-1·d^-1.The positive expression of insulin-like growth factor-1(IGF-1)and insulin-like growth factor-2(IGF-2)in rat ovary was detected by immunohistochemistry after 30 days of drug treatment.The survival rate and proliferation rate of ovarian granulosa cells were measured by MTT,the content of ovarian anti-Mullerian hormone(AMH)was determined by ELISA method,and the content of serum follicle stimulating hormone receptor(FSHR)was detected by PCR.Results:Compared with the blank group,the expression of IGF-1,AMH and FSHR,the survival rate and proliferation of ovarian granulosa cells in the model group decreased significantly(P<0.05,P<0.01).The expression of IGF-1 in the middle and low dose groups of Modified Xiaoyao Powder were significantly decreased(P<0.01),and the AMH and ovarian granulosa cells in low dose group of Modified Xiaoyao Powder were significantly decreased(P<0.05).The expression of IGF-1 and survival rate of ovarian granulosa cells were significantly decreased in bromine group(P<0.05).Compared with the model group,the AMH,IGF-1 and the survival rate of ovarian granulosa cells in the high dose group were significantly increased(P<0.01,P<0.05).The cell proliferation rate and FSHR in the high dose group were significantly higher than those in the model group(P<0.05,P<0.01).Conclusion:High dose group of Modified Xiaoyao Powder can regulate the autocrine/paracrine mechanism of ovary by increasing the contents of IGF-1,FSHR and AMH,and then improve the survival rate of ovarian granulosa cells in animal model.