hsa-miR-145-5p在胰腺癌中的表达及生物信息学分析

目的 研究hsa-miR-145-5p在胰腺癌患者及正常人血浆中的表达模式,探究hsa-miR-145-5p作为胰腺癌诊断标记物的可行性。进一步阐明其在胰腺癌的发生发展过程中可能的调控机制。 方法 采用生物信息学方法进行hsa-miR-145-5p的靶基因预测及功能分析,选择miRanda、TargetScan和RNAhybrid三种软件预测hsa-miR-145-5p的靶基因并结合现有数据库筛选有实验数据支持的靶基因,进一步进行GO功能富集分析,KEGG Pathway富集分析和蛋白互作分析,研究靶基因功能。qPCR验证胰腺癌患者及正常对照组血浆中hsa-miR-145-5p表达。 结果 hsa-miR-145-5p序列在各物种间高度保守;预测其靶基因共得到8 679个,且有实验数据支持靶基因有1 408个;有实验数据支持的靶基因GO富集分析主要显著富集于胞内运输、基因表达调控、细胞内信号传导、细胞生长调控、能量代谢等生物学过程和功能上(FDR<0.01)。KEGG Pathway富集分析靶基因显著富集于p53信号通路、ErbB信号通路、MAPK信号通路、慢性骨髓白血病、膀胱癌、胶质瘤、前列腺癌、胰腺癌等(P<0.01),表明hsa-miR-145-5p在胰腺癌中有重要的调控作用。相较于正常组,患病组hsa-miR-145-5p表达下调。 结论 hsa-miR-145-5p调控多个肿瘤发生相关的基因,在胰腺癌发生相关的生物学过程中具有重要的调控功能,为胰腺癌miRNA标记物的研究提供了线索。 

Expression of hsa-miR-145-5p in pancreatic cancer and its bioinformatics analysis

Objective To investigate the expression pattern of hsa-miR-145-5p in the plasma of patients in pancreatic cancer and normal person,and explore the feasibility of hsa-miR-145-5p as a diagnostic marker for pancreatic cancer,and further clarify its possible regulatory mechanism in the development and progression of pancreatic cancer. Methods Target genes prediction and functional analysis of hsa-miR-145-5p by bioinformatics method.MiRanda,TargetScan and RNAhybrid software were used to predict the target genes of hsa-miR-145-5p and the target genes supported by experimental data,and GO enrichment,KEGG Pathway enrichment analysis and protein interaction analysis were performed to study the function of target genes.RT-qPCR was used to detect the expression of hsa-miR-145-5p in pancreatic cancer and normal control group. Results hsa-miR-145-5p mature sequences were high conserved in several species.The number of target genes were predicted to be 8 679,and the number of target genes that the experimental data supported was 1 408.The GO enrichment analysis of the target genes,which was supported by the experimental data,was mainly concentrated in the biological processes and functions such as intracellular transport,gene expression regulation,intracellular signal transduction,cell growth regulation,energy metabolism,and so on (FDR<0.01).KEGG pathway enrichment analysis was significantly enriched in p53 signaling pathway,ErbB signaling pathway,MAPK signaling pathway,chronic myeloid leukemia,bladder cancer,glioma,prostate cancer,pancreatic cancer,and so on (P<0.01).Compared with the normal group,the expression of hsa-miR-145-5p was down regulated. Conclusion hsa-miR-145-5p regulates multiple tumor related genes,plays an important regulatory role in the biological process of pancreatic cancer and provides clues for the study of a miRNA diagnostic marker in pancreatic cancer.