番茄红素对反式脂肪酸致小鼠肝脏损伤的修复作用

目的 探讨番茄红素(lycopene,LP)对反式脂肪酸(TFA)染毒小鼠肝脏损伤的修复及其可能机制。 方法 SPF级KM小鼠40只(雌雄各半)按体重随机分为正常对照组(生理盐水)、TFA组(50 mg/kg剂量的TFA)、TFA+低、中、高LP组(50 mg/kg剂量的TFA+5 mg/kg、10 mg/kg、20 mg/kg剂量的LP),每组8只(雌雄各半)。隔天进行灌胃染毒,染毒8周。检测丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、总蛋白(TP)、白蛋白(ALB)、甘油三酯(TG)、总胆固醇(TC)、高密度脂蛋白(HDL-C)、低密度脂蛋白(LDL-C)含量及肝组织超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)含量,对肝脏进行病理形态学观察。 结果 与对照组比较,TFA组小鼠体重、肝脏脏器系数、ALT、AST、TG、TC、LDL-C水平及MDA含量增加(P<0.05),TP、ALB、HDL-C含量降低(P<0.05);SOD、GSH-Px活性降低(P<0.05);且其肝组织形态可见肝小叶结构紊乱,可见肝细胞大片坏死。与TFA组相比,TFA+低、中、高LP组体重、肝脏脏器系数、ALT、AST、TG、TC、LDL-C水平及MDA含量降低(P<0.05),TP、ALB、HDL-C含量增加(P<0.05);SOD、GSH-Px活性上升(P<0.05);且肝细胞病理变化明显改善。 结论 番茄红素可修复肝脏细胞,保护肝功能,对反式脂肪酸致小鼠肝损伤有一定的修复作用,其机制可能与番茄红素抗脂质过氧化和清除氧自由基的作用有关。 

Repair effect of Lycopene on liver injury induced by trans fatty acids in mice

Objective To investigate the protection and its probably mechanisms of Lycopene (LP) on the mice with liver injury following trans fatty acid (TFA) damage. Methods Forty KM mice (equivalent males and females) were randomly divided into five groups as follows:normal control group (normal saline), TFA group (50 mg/kg body weight TFA) and groups of TFA with low, middle and high dosages treatment LP protection group (50 mg/kg body weight TFA+5, 10, 20 mg/kg body weight LP), with 8 mice in each group (equivalent males and females). All the experimental procedures were intragastrically applied once every other day, eight weeks in total. The tissues were processed as follows:detection serum ALT, AST, TP, ALB, TG, TC, HDL-C, LDL-C content and liver tissue SOD, GSH-Px activity and MDA content. Results In the groups with TFA, the weight increasing of mice and the organ coefficient of liver, ALT, AST, TP, ALB, TG, TC, LDL-C and MDA content were higher than that in the control group (P<0.05) and TP, ALB, HDL-C content were lower (P<0.05). In the groups with TFA, the activity of liver SOD and GSH-Px was decreased (P<0.05). The livers in the TFA group were damaged to different extents. Compared with the groups with TFA, groups of TFA with low, middle and high dosages plus LP, the weight increasing of mice and the organ coefficient of liver, ALT, AST, TP, ALB, TG, TC, LDL-C and MDA content were lower (P<0.05), and TP, ALB, HDL-C content were higher (P<0.05), the activity of liver SOD and GSH-Px was higher (P<0.05). Following LP treatments, the liver damages in the LP groups with different doses were alleviated. Conclusion LP performs the repairing role for the liver via inhibiting the oxidative stress damages caused by TFA, which mechanisms might be correlated to the effects of anti-lipid peroxidation and oxygen free-radical clearing activity.