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超声法提取土茯苓中总黄酮的工艺研究
引用本文:姚冬梅,刘开蕾,周长青,王锐,吕邵娃,李永吉. 超声法提取土茯苓中总黄酮的工艺研究[J]. 中国中医药信息杂志, 2011, 18(6): 60-62. DOI: 10.3969/j.issn.1005-5304.2011.06.026
作者姓名:姚冬梅  刘开蕾  周长青  王锐  吕邵娃  李永吉
作者单位:1. 黑龙江中医药大学,黑龙江哈尔滨,150040
2. 牡丹江医学院红旗医院二发电分院,黑龙江牡丹江,157000
基金项目:黑龙江中医药管理局中医药科研项目,黑龙江中医药大学科研基金
摘    要:
目的研究超声法提取土茯苓中总黄酮的最佳工艺。方法设计正交试验,采用超声法提取,以总黄酮提取率为指标,对土茯苓中总黄酮的提取工艺进行研究。结果超声法提取土茯苓总黄酮的最佳工艺为:乙醇浓度60%,乙醇用量15倍,提取时间45 min,提取温度60℃。结论该工艺方法简单,耗时短,提取率高。

关 键 词:土茯苓  总黄酮  超声提取  正交试验

Technology Study on Ultrasonic Extraction of Total Flavonoids in Smilax glabra
YAO Dong-mei,LIU Kai-lei,ZHOU Chang-qing,WANG Rui,LV Shao-wa,LI Yong-ji. Technology Study on Ultrasonic Extraction of Total Flavonoids in Smilax glabra[J]. Chinese Journal of Information on Traditional Chinese Medicine, 2011, 18(6): 60-62. DOI: 10.3969/j.issn.1005-5304.2011.06.026
Authors:YAO Dong-mei  LIU Kai-lei  ZHOU Chang-qing  WANG Rui  LV Shao-wa  LI Yong-ji
Affiliation:YAO Dong-mei1,LIU Kai-lei2,ZHOU Chang-qing 2,WANG Rui 1,LV Shao-wa 1,LI Yong-ji 1(1.Heilongjiang University of Chinese Medicine,Harbin 150040,China,2.Red Branch Hospital,the Second Generation,Mudanjiang Medical College,Mudanjiang 157000,China)
Abstract:
Objective To explore the best extraction technique of total flavonoids in Smilax glabra with ultrasonic-assisted technique.Methods The orthogonal design was used in the ultrasonic-assisted technique with the extraction rate of total flavonoids as the index.Results The optimum conditions of the extraction were as follows:the amount of 60%ethanol was 15 times,extracting temperature was 60℃and extracted for 45 min.Conclusion This process was featured by higher extraction rate and shorter extraction time.
Keywords:Smilax glabra  total flavonoids  ultrasonic extraction  orthogonal design  
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