铜绿假单胞菌耐药情况和携带耐药基因分析

目的：分析铜绿假单胞菌（ PA）的耐药情况和携带耐药基因的情况。方法选择2012年6-12月在我院不同来源的50株PA，收集其药敏结果统计分析耐药情况，并用PCR方法检测其携带耐药基因的情况。结果50株细菌中检出7株有TEM基因，6株有OXA-10基因，4株外膜孔蛋白基因OprD2缺失。50株PA菌株对药物的耐药率情况为：亚胺培南6．00％，美罗培南6．52％，多黏菌素7．89％，哌拉西林／他唑巴坦10．42％，左旋氧氟沙星12．77％，阿米卡星14％，环丙沙星14．29％，头孢他啶26．00％，头孢吡肟28．57％，哌拉西林29．55％，庆大霉素30．00％，氨曲南38．00％。结论50株PA菌株中，耐亚胺培南可能是因为OprD2蛋白表达缺失，但并非所有的OprD2缺失均导致亚胺培南耐药。携带TEM和（或） OXA-10基因则可能耐β内酰胺类抗菌药。

Analysis of drug resistance and carrying resistance genes of pseudomonas aeruginosa

Objective To analysis the drug resistance and carrying resistance genes of pseudomonas aeruginosa . Methods Selected 50 strains of pseudomonas aeruginosa from June to December ,2012 in our hospital .Collection susceptibili-ty results,analysis the drug resistance ,the carrying resistance genes was detected by PCR .Results The resistance rates of 50 strains of PA：imipenem 6.0%, meropenem 6.52%, polymyxin 7.89%, piperacillin/tazobactam 10.42%, levofloxacin 12.77%,amikacin 14%,ciprofloxacin 14.29%,ceftazidime 26%,cefepime 28.57%,piperacillin 29.55%,gentamicin 30%, aztreonam 38%.7 strains of PA has TEM gene .6 strains of PA has OXA-10 gene.The outer membrane protein gene of 4 strains of PA wasmissing.Conclusion The missing of OprD2 protein expression deletion may cause the resistant to imipenem .But,not all OprD2 deletions have led to imipenem .TEM and（or）OXA gene may resistance to the β-lactam antibiotic.