| 用高效液相色谱法测定冬虫夏草及虫草乌鸡胶丸中麦角甾醇的含量 |
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| 引用本文： | 李云华,李修禄.用高效液相色谱法测定冬虫夏草及虫草乌鸡胶丸中麦角甾醇的含量[J].药学学报,1991(10). |
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| 作者姓名： | 李云华 李修禄 |
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| 作者单位： | 第二军医大学药学院，第二军医大学药学院 上海 200433 1989届硕士研究生，上海 200433 |
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| 摘 要： | 本文将样品直接皂化,环己烷提取,用分配柱和吸附柱进行提取液的净化和浓缩,HPLC法测定了冬虫夏草及虫草乌鸡胶丸中麦角甾醇的含量,为虫草及其制剂中麦角甾醇的含量测定建立了简便可行的方法。
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| 关 键 词： | 高效液相色谱法 麦角甾醇 冬虫夏草 蛹虫草 人工虫草 虫草乌鸡胶丸 |
DETERMINATION OF ERGOSTEROL IN CORDYCEPS SINENSIS AND CORDYCEPS BLACK-BONE CHICKEN CAPSULES BY HPLC |
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| YH Li and XL Li.DETERMINATION OF ERGOSTEROL IN CORDYCEPS SINENSIS AND CORDYCEPS BLACK-BONE CHICKEN CAPSULES BY HPLC[J].Acta Pharmaceutica Sinica,1991(10). |
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| Authors: | YH Li and XL Li |
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| Abstract: | A high performance liquid chromatographic method (HPLC)was developed for the determination of ergosterol in Cordyceps sinensis and Cordyceps black-bone chicken capsules. The sample was mixed with 0.5 ml of saturated KOH solution and 5 ml of ethanol, and refluxed for 1h. The saponified mixture was diluted with 5ml of 10% NaCl solution and extracted 3 times with 5 ml portions of cyclohexane. The cyclohexane extracts were purified by partition column and concentrated by adsorption column and then analysed by HPLC. The other conponents of the Cordyceps black- bone chicken capsules have no interference to the determination of ergosterol. The proposed method is rapid, precise, accurate and sensitive. The whole process can be done in 2h. |
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| Keywords: | HPLC Ergosterol Cordyceps sinensis Cordyceps militaris Cultured Cordyceps Cordyceps black- bone chicken capsule |
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