Effect of preparation methods on the assessment of airborne concentrations of asbestos fibres by transmission electron microscopy |
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| Affiliation: | 1. Institut National de Recherche et de Sécurité B.P. No. 27, Avenue de Bourgogne, 54501 Vandoeuvre-les-Nancy, France;2. Laboratoire d''Etude des Particules Inhalées, 11 rue George Eastman, 75013 Paris, France;1. Department of Chemical Engineering, University of Utah, Salt Lake City, USA;2. Department of Biomedical Engineering, University of Utah, Salt Lake City, USA;1. Dept. of Experimental Molecular Imaging, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany;2. Dept. of Tissue Engineering & Textile Implants, Applied Medical Engineering, Helmholtz Institute for Biomedical Engineering, RWTH Aachen University, Pauwelsstrasse 20, 52074 Aachen, Germany;3. Institut für Textiltechnik, RWTH Aachen University, Otto-Blumenthal-Strasse 1, 52074 Aachen, Germany;4. Institute for Molecular Cardiovascular Research, University Clinic, RWTH Aachen University, Pauwelsstrasse 30, 52074 Aachen, Germany;5. Dept. of Controlled Drug Delivery, University of Twente, PO Box 217, 7500 AE Enschede, The Netherlands;1. Department of Chemical and Geological Sciences, University of Modena and Reggio Emilia, Via Campi 103, I-41125 Modena, Italy;2. ESRF, 71 Avenue de Martyrs, F-38000 Grenoble, France;3. Cancer Research Centre, European Ramazzini Institute, Castello di Bentivoglio, Via Saliceto 3, I-40010 Bentivoglio, Bologna, Italy;4. Institut für Geowissenschaften Mineralogie, Friedrich-Schiller-Universität Jena, Carl-Zeiss-Promenade 10, D-07745 Jena, Germany;5. Laboratory for Material Chemistry, National Institute of Chemistry, Hajdrihova 19, Ljubljana, Slovenia;1. Chair of Materials Science, Otto Schott Institute of Materials Research (OSIM), Friedrich Schiller University Jena, Löbdergraben 32, D-07743 Jena, Germany;2. Institute for Bioprocessing and Analytical Measurement Techniques, Rosenhof 1, D-37308 Heilbad Heiligenstadt, Germany;3. Department of General, Visceral and Vascular Surgery, Jena University Hospital, Erlanger Allee 101, D-07747 Jena, Germany;4. Jena Center for Soft Matter, Friedrich Schiller University Jena, Humboldstr. 10, D-07743 Jena, Germany;5. Jena School for Microbial Communication (JSMC), Friedrich Schiller University Jena, Neugasse 23, D-07743 Jena, Germany |
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| Abstract: | ![]()
The usual indirect and direct preparation methods used to assess the airborne asbestos fibre concentrations by transmission electron microscopy (TEM) have been investigated in detail. It has been shown that ultrasonics, often used in the indirect preparation method to facilitate the recovery of dust, may significantly increase the fibre number concentration. This effect is highly variable and dependent on the characteristics of the workplace where the samples were collected. If the indirect preparation method is modified to avoid the use of the ultrasonic treatment, both the direct and indirect preparation methods give comparable results for fibres of length greater than 5 μm. By contrast, the number concentrations of fibres shorter than 5 μm are always different but the reason for this is not clear. |
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